Quantitative Detection of Pathogen DNA of Verticillium Wilt on Smoke Tree Cotinus coggygria.
Quantitative Detection of Pathogen DNA of Verticillium Wilt on Smoke Tree Cotinus coggygria.
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DOI：
10.1094/pdis-04-13-0406-re
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发表时间：
2013-11
期刊：
影响因子：
4.5
通讯作者：
Yonglin Wang;Yan Wang;C. Tian
中科院分区：
文献类型：
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作者：
Yonglin Wang;Yan Wang;C. Tian
大丽轮枝菌（Verticilliumdahliae）是一种广泛存在的土传真菌，是引起北京香山公园红叶景观的主要病原菌。在这项研究中，我们检测真菌的存在的基础上的真菌DNA在植物和土壤中的数量，通过使用定量巢式实时聚合酶链反应（QNRT-PCR）。QNRT-PCR检测结果对大丽轮枝菌具有高度特异性，可以检测不同植物组织中随时间的枯萎病动态。在受侵染的土壤中进行的QNRT-PCR试验表明，土壤接种密度低至1微菌核/g土壤。QNRT-PCR结果显示，病原菌DNA含量与黄萎病严重度等级之间存在较强的相关性，表明可在种植前对棉花黄萎病菌土壤接种物进行定量评估。这些数据表明，QNRT-PCR是一种敏感、可靠的监测植物和土壤中的土传病原菌大丽轮枝菌的方法。本研究结果可为制定新的黄萎病防治措施和烟树种植前的黄萎病风险评估提供参考。
Verticillium dahliae is a ubiquitous soilborne fungus and the causal agent of smoke tree vascular wilt, which presents a major threat to the famous "red-leaf" scenery of the Fragrant Hills Park in Beijing, China. In this study, we detected the presence of the fungus based on the amount of fungal DNA in planta and in the soil by using quantitative nested real-time polymerase chain reaction (QNRT-PCR). The QNRT-PCR assay results were highly specific for V. dahliae and could detect disease wilt dynamics over time in different plant tissues. Tests with QNRT-PCR in infested soils showed the detection of soil inoculum densities as low as 1 microsclerotium/g of soil. The QNRT-PCR data showed strong correlation between the quantity of pathogen DNA and the Verticillium wilt disease severity rating, suggesting that quantification of V. dahliae soil inoculum could be conducted to assess Verticillium wilt risk before planting. These data indicate that QNRT-PCR is a sensitive and reliable method to monitor the soilborne pathogen V. dahliae in planta and in soil. The results of this study can be useful in the development of new disease control measures for Verticillium wilt and assessment of the risk of V. dahliae infection of smoke tree before planting.