A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR
A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR
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DOI：
10.1039/d0cc03612a
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发表时间：
2020-10-07
影响因子：
4.9
通讯作者：
Ortega-Roldan, Jose L.
中科院分区：
文献类型：
--
作者：
Medina-Carmona, Encarnacion;Varela, Lorena;Ortega-Roldan, Jose L.
与膜相互作用的膜蛋白和化合物的活性由周围的脂质组成调节。然而，没有简单的方法来确定真核系统中这些环状磷脂的组成。在本文中，我们描述了一种简单的方法，该方法能够使用SMA-纳米盘和常规（1）H-P-31 NMR鉴定和定量膜相关化合物周围的脂质组成。
The activity of membrane proteins and compounds that interact with the membrane is modulated by the surrounding lipid composition. However, there are no simple methods that determine the composition of these annular phospholipids in eukaryotic systems. Herein, we describe a simple methodology that enables the identification and quantification of the lipid composition around membrane-associated compounds using SMA-nanodiscs and routine(1)H-P-31 NMR.