Functional screen for microRNAs of Nilaparvata lugens reveals that targeting of glutamine synthase by miR-4868b regulates fecundity
Functional screen for microRNAs of Nilaparvata lugens reveals that targeting of glutamine synthase by miR-4868b regulates fecundity
复制标题
对褐飞虱 microRNA 的功能筛选揭示了 miR-4868b 靶向谷氨酰胺合酶可调节繁殖力
DOI：
10.1016/j.jinsphys.2015.11.003
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发表时间：
2015-12-01
影响因子：
2.2
通讯作者：
Zhang, Wenqing
中科院分区：
文献类型：
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作者：
Fu, Xian;Li, Tengchao;Zhang, Wenqing
昆虫生殖力受基因型和环境的互作调节。microRNAs（miRNAs）也通过调节参与这些生理过程的基因而在昆虫发育和生殖中起作用。尽管已经鉴定了数百种昆虫miRNAs，但大多数的生物学作用仍然知之甚少。在这里，我们使用了多算法的方法来预测在褐飞虱（BPH）的生殖力相关基因的3 'UTR中的miRNA靶标，并确定了38个假定的miRNA靶向9个生殖力相关基因。选择高排名的miRNA用于靶标验证。在S2细胞中使用双荧光素酶报告基因测定，我们实验验证了N。在一些实施方案中，本发明提供了一种将褐藻胶谷氨酰胺合成酶（NIGS）作为microRNA-4868 b（miR-4868 b）的真实靶标的方法。在雌性中，NIGS蛋白表达在注射miR-4868 b模拟物后下调，但在注射miR-4868 b抑制剂后上调。此外，miR-4868 b的过表达降低了N. lugens。这些发现表明miR-4868 b参与调节N.通过靶向NIGS提高褐飞虱的繁殖力此外，这项研究可能会导致更好地了解这种重要的农业害虫的繁殖力。(C)2015爱思唯尔有限公司版权所有。
Insect fecundity is regulated by the interaction of genotypes and the environment. MicroRNAs (miRNAs) also act in insect development and reproduction by regulating genes involved in these physiological processes. Although hundreds of insect miRNAs have been identified, the biological roles of most remain poorly understood. Here, we used a multi-algorithm approach for miRNA target prediction in 3'UTRs of fecundity-related genes in the brown planthopper (BPH) Nilaparvata lugens and identified 38 putative miRNAs targeting 9 fecundity-related genes. High-ranked miRNAs were selected for target validation. Using a dual luciferase reporter assay in S2 cells, we experimentally verified N. lugens glutamine synthetase (NlGS) as an authentic target of microRNA-4868b (miR-4868b). In the females, NlGS protein expression was down-regulated after injection of a miR-4868b mimic but up-regulated after injection of a miR-4868b inhibitor. In addition, overexpression of miR-4868b reduced fecundity, and disrupted ovary development and Vg expression in N. lugens. These findings showed that miR-4868b is involved in regulating N. lugens fecundity by targeting NlGS. Moreover, this study may lead to better understanding of the fecundity of this important agricultural insect pest. (C) 2015 Elsevier Ltd. All rights reserved.