Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.
Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.
复制标题
通过循环流系统进行温度跳跃实时核磁共振监测蛋白质折叠动力学。
作者：
K. Yamasaki;Yuji Obara;M. Hasegawa;Hideki Tanaka;T. Yamasaki;T. Wakuda;M. Okada;T. Kohzuma
开发了一种核磁共振方法，可以实时监测由温度跃变引起的反应(大约几秒钟)。在循环流系统中，热水浴和冷水浴被整合在一起，后者在核磁共振探头内。核糖核酸酶A的重折叠反应是由快速冷却引发的，并由12 S的一系列核磁共振测量监测到的。数据经主成分分析处理，成功地将与结构变化有关的因子与基线不稳定和/或噪声有关的因子分离出来，其指数速率常数为0.20-0.7S(-1)。速率常数的温度依赖性揭示了复性反应过渡态的形成是由熵驱动的。
An NMR method was developed that allows for real-time monitoring of reactions (on the order of seconds) induced by a temperature jump. In a recycle flow system, heating and cooling baths were integrated, with the latter inside the NMR probe. A refolding reaction of ribonuclease A was triggered by rapid cooling and monitored by a series of NMR measurements over 12 s. Data were processed by principal component analysis, in which a factor related to the structural change with an exponential rate constant of 0.2-0.7 s(-1) was successfully separated from factors related to baseline instability and/or noise. Temperature dependency of the rate constant revealed the entropy-driven formation of the transition state of the refolding reaction.
通过连续循环流对牛胰腺核糖核酸酶 A 的折叠中间体进行结构研究。
影响因子：
2.9
作者：
Adler,M;Scheraga,HA
通讯作者：
Scheraga,HA
核糖核酸酶 A 折叠过程中通过酰胺质子交换捕获的结构中间体。
影响因子：
2.9
作者：
Kim,PS;Baldwin,RL
通讯作者：
Baldwin,RL