Cytotoxic and genotoxic effects of multi-wall carbon nanotubes on human umbilical vein endothelial cells in vitro
Cytotoxic and genotoxic effects of multi-wall carbon nanotubes on human umbilical vein endothelial cells in vitro
复制标题
多壁碳纳米管对人脐静脉内皮细胞的体外细胞毒性和基因毒性作用
DOI：
10.1016/j.mrgentox.2011.01.014
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发表时间：
2011-04-03
影响因子：
1.9
通讯作者：
Zhu, Xin-Qiang
中科院分区：
文献类型：
--
作者：
Guo, Yuan-Yuan;Zhang, Jun;Zhu, Xin-Qiang
碳纳米材料在各个领域有着广泛的应用。然而，有研究表明，暴露于纳米颗粒可能会导致血管内皮细胞损伤和功能障碍，从而导致血管疾病的发生。本研究旨在探讨多壁碳纳米管(MWCNTs)对人脐静脉内皮细胞(HUVECs)的细胞毒性和遗传毒性作用。光学和透射电子显微镜研究表明，多壁碳纳米管能够快速进入细胞，分布在细胞质和胞内的囊泡中，并引起细胞形态的改变。多壁碳纳米管可降低人脐静脉内皮细胞的存活率，并诱导其发生凋亡。更重要的是。多壁碳纳米管可引起DNA损伤，表现为γ-H_2AX焦点的形成。多壁碳纳米管还影响细胞的氧化还原状态，如增加细胞内活性氧(ROS)和丙二醛(MDA)水平，以及改变超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-Px)水平。而自由基清除剂N-乙酰-L-半胱氨酸(NAC)预先孵育可抑制多壁碳纳米管的细胞毒性和遗传毒性效应。综上所述，这些结果表明，多壁碳纳米管可能通过氧化损伤途径诱导HUVECs产生细胞毒性和遗传毒性效应。(C)2011爱思唯尔B.V.保留所有权利。
Carbon nanomaterials have multiple applications in various areas. However, it has been suggested that exposure to nanoparticles may be a risk for the development of vascular diseases due to injury and dysfunction of the vascular endothelium. Therefore, in the present study, the cytotoxic and genotoxic effects of multi-wall carbon nanotubes (MWCNTs) on human umbilical vein endothelial cells (HUVECs) were evaluated. Optical and transmission electronic microscopy (TEM) study showed that MWCNTs were able to enter cells rapidly, distribute in the cytoplasm and intracellular vesicles and induce morphological changes. Exposure to MWCNTs reduced the viability of HUVECs, and induced apoptosis in HUVECs. Furthermore. MWCNTs could cause DNA damage as indicated by the formation of gamma H2AX foci. MWCNTs also affected cellular redox status, e.g., increasing intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as altering superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) levels. On the other hand, the free radical scavenger N-acetyl-L-cysteine (NAC) preincubation can inhibit the cytotoxic and genotoxic effects of MWCNTs. Taken together, these results demonstrated that MWCNTs could induce cytotoxic and genotoxic effects in HUVECs, probably through oxidative damage pathways. (C) 2011 Elsevier B.V. All rights reserved.