DISTRIBUTION OF BLOOD (FE-59) AND PLASMA (I-131) VOLUMES OF RATS DETERMINED BY LIQUID NITROGEN FREEZING
DISTRIBUTION OF BLOOD (FE-59) AND PLASMA (I-131) VOLUMES OF RATS DETERMINED BY LIQUID NITROGEN FREEZING
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DOI：
10.1161/01.res.4.4.419
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发表时间：
1956-01-01
影响因子：
20.1
通讯作者：
LASHER, EP
中科院分区：
文献类型：
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作者：
EVERETT, NB;SIMMONS, B;LASHER, EP
测定冷冻成年大鼠及其各主要器官或组织的红细胞体积、血浆体积和组织红细胞压积。血容量以微升/克器官或体重表示。采用同位素稀释法，使用Fe 59标记的红细胞进行红细胞测定，使用I131血清白蛋白进行血浆测定。将动物冷冻在液氮中，取出器官并在无失血的情况下进行测定。雌性大鼠的平均血容量为53.1 [mu]l/g（C. V. 1.5），体血细胞比容为36。雄性的相应值为55.6 [mu]l/g（C. V. 2.3）和红细胞压积36.7。器官血容量范围从肺的517 [mu]l/g到睾丸的15.6 [mu]l/g。提供了22种不同器官和组织的组织红细胞压积和单位重量总血量。
The red cell volume, plasma volume and the tissue hema-tocrit were determined for the frozen adult rat and for each of its major organs or tissues. Blood volumes were expressed in microliters per gram of organ or body weight. The isotope dilution method was applied using Fe59 tagged erythrocytes for the red cell determinations and I131 serum albumin for the plasma determinations. Animals were frozen in liquid N and organs were removed and assayed without blood loss. The mean blood volume of female rats is 53.1 [mu]l/g (C.V. 1.5) with a body hematocrit of 36. The corresponding value for males is 55.6 [mu]l/g (C. V. 2.3) and a hematocrit of 36.7. The organ blood volumes range from a high of 517 [mu]l/g for the lung to a low of 15.6 [mu]l/g for the testis. The tissue hematocrits and the total blood volumes per unit weight of 22 different organs and tissues are presented.