The complete mitochondrial genomes sequences of Asio flammeus and Asio otus and comparative analysis
The complete mitochondrial genomes sequences of Asio flammeus and Asio otus and comparative analysis
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DOI：
10.1360/04yc0117
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发表时间：
2004-12-01
期刊：
影响因子：
--
通讯作者：
Li, QW
中科院分区：
文献类型：
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作者：
Sun, Y;Ma, F;Li, QW
对火种亚洲狮和美洲狮的线粒体全基因组进行了测序，发现其全长分别为18858 bp和18493 bp。令人惊讶的是，前者是迄今为止测序的所有鸟类线粒体基因组中最大的。这两个基因组与Gallus Gallus的基因序列非常相似，都不包含伪控制区，但在ND3基因174位都有一个额外的碱基，即Cytidine。红火亚洲鼠（Asio flammeus）和野亚洲鼠（Asio otus）线粒体基因组控制区长度分别为3288 bp和2926 bp，是脊椎动物中最长的，是除粘粘动物（Myxine glutinosa）之外最长的，这也是两个基因组规模较大的原因。Asio flammeus和Asio otus的控制区3'端包含许多串联重复序列，这些序列与假定的控制元件Mt5高度相似，并可能形成稳定的茎环二级结构。这些重复序列可能在调节线粒体基因组的转录和复制中起重要作用。本研究结果可能为揭示线粒体基因组的起源和进化机制提供重要线索。
The complete mitochondrial genomes of Asio flammeus and Asio otus were sequenced and found to span 18858 bp and 18493 bp, respectively. It is surprising to find the former to be the largest among all avian mitochondrial genomes sequenced so far. The two genomes have very similar gene order with that of Gallus gallus, neither contains the pseudo control region, but both have a single extra base, namely Cytidine, at position 174 in ND3 gene. The control regions of Asio flammeus and Asio otus' mitochondrial genomes span 3288 bp and 2926 bp respectively, which are the longest among vertebrates except for Myxine glutinosa and contribute to the large size of two genomes. The 3' end of the control region of Asio flammeus and Asio otus contains many tandemly repeated sequences, which are highly similar to a putative control element, i.e. Mt5, and may form stable stem-loop secondary structures. Such repeated sequences probably play an important role in regulating transcription and replication of mitochondrial genome. Our results may provide important clues for uncovering the origin and evolution mechanisms of mitochondrion genome.