A GRISM-based probe for spectrally encoded confocal microscopy.
A GRISM-based probe for spectrally encoded confocal microscopy.
复制标题
用于光谱编码共焦显微镜的基于 GRISM 的探针。
作者：
C. Pitris;B. Bouma;M. Shiskov;G. Tearney
光谱编码共聚焦显微镜（SECM）是一种获得高分辨率、浑浊样品中微观结构深度切片图像的新方法。通过在波长中编码一个空间维度，与标准扫描共聚焦显微镜相比，成像探针可以大大简化，有可能实现内窥镜。然而，使用衍射光栅进行光谱编码会使光轴穿过探头，从而使窄直径仪器的设计复杂化。在本文中，我们描述了一种基于高折射率棱镜和透射全息光栅的单光轴元件的新用途，用于设计窄直径SECM器件。用10.0 mm探针获得的共聚焦图像显示横向分辨率为1.1微米，视野为650微米。
Spectrally encoded confocal microscopy (SECM) is a novel approach for obtaining high resolution, depth-sectioned images of microstructure within turbid samples. By encoding one spatial dimension in wavelength, imaging probes can be greatly simplified compared to standard scanning confocal microscopes, potentially enabling endoscopic implementation. The use of a diffraction grating for spectral encoding, however, skews the optical axis through the probe, thus complicating the design of narrow diameter instruments. In this Letter, we describe a novel use of a single-optical-axis element based on high index-of-refraction prisms and a transmission holographic grating for the design of narrow diameter SECM devices. Confocal images obtained with a 10.0 mm probe demonstrate a transverse resolution of 1.1 microm and a field of view of 650 microm.