Low-frequency oscillations of the laser Doppler perfusion signal in human skin
Low-frequency oscillations of the laser Doppler perfusion signal in human skin
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DOI：
10.1016/j.mvr.2006.05.006
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发表时间：
2006-11-01
影响因子：
3.1
通讯作者：
Kirkeboen, Knut Arvid
中科院分区：
文献类型：
--
作者：
Kvandal, Per;Landsverk, Svein Aslak;Kirkeboen, Knut Arvid
对激光多普勒血流（LDF）信号在0.0095 ~ 2.0 Hz的频率区间进行频谱分析，发现血流振荡频率在1.0、0.3、0.1、0.04和0.01 Hz左右。心跳、呼吸、血管平滑肌固有的肌源性活动、血管壁和血管内皮的神经源性活动分别影响这些振荡。本研究的第一个目的是研究是否可以在人体皮肤血液灌注信号的0.0095 Hz以下的频率区域检测到慢振荡成分。用内皮依赖性血管扩张剂乙酰胆碱（ACh）和内皮依赖性血管扩张剂硝普钠（SNP）对健康男性受试者进行离子导入时未受刺激的基底血皮肤灌注和增强灌注，并计算小波变换。在基础条件和ACh和SNP离子导入过程中，均存在0.005 ~ 0.0095 Hz的低频振荡。与SNP相比，乙酰胆碱离子导入使归一化幅度增加的程度更大（P = 0.001），表明受血管内皮的调节。为了进一步了解这种内皮依赖性的机制，我们用n - g -单甲基- l-精氨酸（L-NMMA）抑制一氧化氮（NO）的合成，用阿司匹林抑制前列腺素（PG）的合成。在0.005-0.0095 hz区间，L-NNIMA对ACh和SNP离子导入的反应增加没有影响（P = 0.006），但在0.0095-0.02 1 hz区间，L-NNIMA消除了差异（P = 0.97）。在两个频率区间内，阿司匹林对乙酰胆碱和SNP的反应差异没有影响。因此，其他内皮机制，如内皮衍生的超极化因子（EDHF），可能参与了第六频率间隔（0.005-0.0095 Hz）的调节。(c) 2006爱思唯尔公司版权所有。
Spectral analysis of the laser Doppler flow (LDF) signal in the frequency interval from 0.0095-2.0 Hz reveals blood flow oscillations with frequencies around 1.0, 0.3, 0.1, 0.04 and 0.01 Hz. The heartbeat, the respiration, the intrinsic myogenic activity of vascular smooth muscle, the neurogenic activity of the vessel wall and the vascular endothelium influence these oscillations, respectively. The first aim of this study was to investigate if a slow oscillatory component could be detected in the frequency area below 0.0095 Hz of the human cutaneous blood perfusion signal. Unstimulated basal blood skin perfusion and enhanced perfusion during iontophoresis with the endothelium-dependent vasodilator acetylcholine (ACh) and the endothelium-independent vasodilator sodium nitroprusside (SNP) were measured in healthy male volunteers and the wavelet transform was computed. A low-frequency oscillation between 0.005 and 0.0095 Hz was found both during basal conditions and during iontophoresis with ACh and SNP. Iontophoresis with ACh increased the normalized amplitude to a greater extent than SNP (P = 0.001) indicating modulation by the vascular endothelium. To gain further insight into the mechanisms for this endothelium dependency, we inhibited nitric oxide (NO) synthesis with N-G-monomethyl-L-arginine (L-NMMA) and prostaglandin (PG) synthesis by aspirin. L-NNIMA did not affect the increased response to ACh vs. SNP iontophoresis in the 0.005-0.0095-Hz interval (P = 0.006) but abolished the difference in the 0.0095-0.02 1-Hz interval (P = 0.97). Aspirin did not affect the difference in response to ACh and SNP in either of the two frequency intervals. Thus, other endothelial mechanisms, such as endothelium-derived hyperpolarizing factor (EDHF), might be involved in the regulation of this sixth frequency interval (0.005-0.0095 Hz). (c) 2006 Elsevier Inc. All rights reserved.