DNA Methylation Status of the IL8 Gene Promoter in Aggressive Periodontitis
DNA Methylation Status of the IL8 Gene Promoter in Aggressive Periodontitis
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DOI：
10.1902/jop.2010.100082
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发表时间：
2010-09-01
影响因子：
4.3
通讯作者：
de Souza, Ana P.
中科院分区：
文献类型：
--
作者：
Andia, Denise C.;de Oliveira, Naila F. P.;de Souza, Ana P.
背景：评估细胞因子基因甲基化状态的研究可能与某些细胞因子表达改变的炎症性疾病有关，如牙周炎。本研究观察了广泛性侵袭性牙周炎（AgP）患者口腔上皮细胞中白细胞介素-8 （il -8）基因启动子的DNA甲基化状态，并与对照组进行了比较。方法：对37例广泛性AgP患者和37例对照组口腔上皮细胞的基因组DNA进行纯化和亚硫酸氢钠修饰。修饰后的DNA通过甲基化特异性聚合酶链反应提交，在10%聚丙烯酰胺凝胶上电泳，并染色。结果：出现广泛性AgP的受试者口腔上皮细胞中IL8基因启动子低甲基化的频率高于对照组（广泛性AgP组为86.5%，对照组为62%；P = 0.016; chi(2)检验）。结论：与对照组相比，广泛性AgP患者的口腔上皮细胞在il - 8基因启动子区域存在显著的低甲基化状态。这种低甲基化状态可能反映了口腔上皮细胞的普遍状况，包括牙龈上皮，因为在使用漱口水期间也收集了牙龈上皮细胞。中华牙周病杂志（英文版）；2010;31(1):444 - 444。
Background: Studies evaluating the methylation status of cytokine genes may have relevance for inflammatory diseases in which the expression of some cytokines is altered, such as periodontitis. This study observes the DNA methylation status in the interleukin-8 (IL8) gene promoter in cells of the oral epithelium of subjects affected by generalized aggressive periodontitis (AgP) and compares it to those of control subjects.Methods: Genomic DNA from epithelial oral cells of 37 generalized AgP patients and 37 controls were purified and modified by sodium bisulphite. Modified DNA was submitted by methylation-specific polymerase chain reaction, electrophoresed on 10% polyacrylamide gels, and stained.Results: Subjects who presented generalized AgP have a higher frequency of hypomethylation of the IL8 gene promoter in oral epithelium cells than that of controls (86.5% in the generalized AgP group versus 62% in the control group; P = 0.016; chi(2) test).Conclusions: A marked hypomethylated status is found in the oral epithelial cells of subjects presenting with generalized AgP, compared to controls, in the promoter region of the IL8 gene. This hypomethylated status may reflect a generalized condition of oral epithelial cells, including gingival epithelium, because gingival epithelial cells were also collected during mouthwash use. J Periodontol 2010;81:1336-1341.