Temperature dependence of parasitic infection and gut bacterial communities in bumble bees
Temperature dependence of parasitic infection and gut bacterial communities in bumble bees
复制标题
大黄蜂寄生虫感染和肠道细菌群落的温度依赖性
DOI：
10.1111/1462-2920.14805
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发表时间：
2019
影响因子：
5.1
通讯作者：
McFrederick, Quinn S.
中科院分区：
文献类型：
--
作者：
Palmer‐Young, Evan C.;Ngor, Lyna;Burciaga Nevarez, Rodrigo;Rothman, Jason A.;Raffel, Thomas R.;McFrederick, Quinn S.
高温（如发烧）和肠道菌群都能影响宿主对感染的抵抗力。然而，温度驱动的肠道菌群变化对寄生虫耐药性的影响仍未被探索。我们研究了感染虫锥虫的大黄蜂的感染和肠道细菌群落的温度依赖性。在21至37°C期间，感染强度下降了80%以上。感染峰值温度低于体外生长预测，这与寄主、寄生虫和肠道共生体热性能曲线的不匹配一致。肠道细菌群落大小和组成对温度表现出轻微但显著的非线性和分类群特异性响应。在以往的研究中与感染呈负相关的总肠道细菌丰度和Orbaceae丰度与感染呈正相关。含有蜜蜂病原体的肠杆菌科细菌的流行率随着温度的升高而下降，这表明高温可能对多种肠道病原体具有保护作用。我们的研究结果表明，对感染的抗性不仅反映了宿主和寄生虫性能的温度依赖性，还反映了肠道细菌的温度依赖性活性。肠道寄生虫-共生体相互作用的热生态学可能与传染病广泛相关，无论是在气候变化的变温生物中，还是在表现出发热免疫的恒温动物中。
High temperatures (e.g., fever) and gut microbiota can both influence host resistance to infection. However, effects of temperature‐driven changes in gut microbiota on resistance to parasites remain unexplored. We examined the temperature dependence of infection and gut bacterial communities in bumble bees infected with the trypanosomatid parasiteCrithidia bombi. Infection intensity decreased by over 80% between 21 and 37°C. Temperatures of peak infection were lower than predicted based on parasite growthin vitro, consistent with mismatches in thermal performance curves of hosts, parasites and gut symbionts. Gut bacterial community size and composition exhibited slight but significant, non‐linear, and taxon‐specific responses to temperature. Abundance of total gut bacteria and of Orbaceae, both negatively correlated with infection in previous studies, were positively correlated with infection here. Prevalence of the bee pathogen‐containing family Enterobacteriaceae declined with temperature, suggesting that high temperature may confer protection against diverse gut pathogens. Our results indicate that resistance to infection reflects not only the temperature dependence of host and parasite performance, but also temperature‐dependent activity of gut bacteria. The thermal ecology of gut parasite‐symbiont interactions may be broadly relevant to infectious disease, both in ectothermic organisms that inhabit changing climates, and in endotherms that exhibit fever‐based immunity.