Detection of 5-methylcytosine removal from DNA of Friend erythroleukemia cells following exposure to several differentiating agents.
Detection of 5-methylcytosine removal from DNA of Friend erythroleukemia cells following exposure to several differentiating agents.
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检测 Friend 红白血病细胞暴露于几种分化剂后 DNA 中 5-甲基胞嘧啶的去除情况。
DOI：
10.1016/0304-3835(89)90191-2
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发表时间：
1989
期刊：
影响因子：
9.7
通讯作者：
Cox,R
中科院分区：
文献类型：
--
作者：
Kuykendall,JR;Cox,R
利用~（32）P-标记方法可以监测Friend红白血病细胞（FELC）分化过程中DNA中5-甲基胞嘧啶（5-mC）的稳定性。对照细胞的现有DNA中的5-mC水平保持恒定20小时，在近3.95%的总标记的胞嘧啶。将预标记细胞暴露于5 mM N′-甲基烟酰胺（N′-MN）、4 mM N-甲基二乙酰胺（HMBA）或270 mM二甲亚砜（DMSO）相同时间段内，导致DNA现有部分中存在的标记胞嘧啶池中的5-mC分别损失6.1%、4.8%和1.8%。
The use of a32P-labeling procedure has allowed us to monitor the stability of 5-methylcytosine (5-mC) in the existing fraction of DNA in differentiating Friend erythroleukemia cells (FELCs). The levels of 5-mC in existing DNA of control cells remained constant for 20 h, at nearly 3.95% of total labeled cytosine. Exposure of pre-labeled cells to 5 mM N′-methylnicotinamide (N′-MN), 4 mM hexamethylene bisacetamide (HMBA), or 270 mM dimethylsulfoxide (DMSO) over the same time-period resulted in the loss of 6.1, 4.8 and 1.8%, respectively, of the 5-mC from the pool of labeled cytosine present in the existing fraction of the DNA.