DYNEIN FAMILY OF MOTOR PROTEINS - PRESENT STATUS AND FUTURE QUESTIONS
DYNEIN FAMILY OF MOTOR PROTEINS - PRESENT STATUS AND FUTURE QUESTIONS
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DOI：
10.1002/cm.970320214
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发表时间：
1995-01-01
影响因子：
--
通讯作者：
GIBBONS, IR
中科院分区：
文献类型：
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作者：
GIBBONS, IR
动力蛋白重链序列关系的分析表明，动力蛋白马达蛋白包括一个单一的同源家族，三个主要分支，细胞质动力蛋白，轴丝动力蛋白，和第三个分支代表DYH 1B，位于其他两个之间。在该家族的所有分支中，动力蛋白重链具有四个拷贝的P-环基序，用于核苷酸结合位点，在其中间区间隔约300个残基，在水解ATP结合位点的P-环中具有氨基酸序列GPAGTGKT。细胞质动力蛋白看起来更原始，因为重链通常以同源二聚体的形式出现，其四个P环基序通过基因复制的早期进化痕迹是可识别的。在轴丝亚家族中，重链作为由多个基因编码的异源二聚体或异源三聚体出现，并且它们的非水解P环基序更加发散，几乎没有通过基因复制的起源的痕迹。DYH 1B亚家族在序列上与细胞质动力蛋白更密切相关，但在功能上似乎与轴丝动力蛋白相关，因为它在重新合成期间上调并且尚未在生物体中发现，如酵母和网囊藻，它们完全没有纤毛或鞭毛。(C)1995年Wiley-Liss，Inc.
Analysis of sequence relationships in dynein heavy chains shows that dynein motor proteins comprise a single homologous family with three main branches, cytoplasmic dynein, axonemal dynein, and a third branch represented by DYH1B that lies between the other two. In all branches of the family the dynein heavy chain has four copies of the P-loop motif for a nucleotide-binding site spaced similar to 300 residues apart in its midregion, with the amino acid sequence GPAGTGKT in the P-loop of the hydrolytic ATP-binding site. Cytoplasmic dyneins appear more primitive in that the heavy chain usually occurs as a homodimer, with traces of the early evolution of its four P-loop motifs by gene duplication being recognizable. In the axonemal subfamily the heavy chains occur as heterodimers or heterotrimers encoded by multiple genes, and their non-hydrolytic P-loop motifs are much more divergent with little trace of their origin by gene duplication, The DYH1B subfamily is more closely related to the cytoplasmic dyneins in sequence, but appears related to axonemal dyneins in function since it becomes upregulated during reciliation and has not been found in organisms, such as yeast and Dictyostelium, that are totally without cilia or flagella. (C) 1995 Wiley-Liss, Inc.