Characterization of Polymeric Microcapsules Containing a Low Molecular Weight Peptide for Controlled Release
Characterization of Polymeric Microcapsules Containing a Low Molecular Weight Peptide for Controlled Release
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DOI：
10.1017/s143192761201389x
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发表时间：
2013-02-01
影响因子：
2.8
通讯作者：
Potts, Jay D.
中科院分区：
文献类型：
--
作者：
Moore, Keith;Amos, Jennifer;Potts, Jay D.
需要延长快速代谢的低分子量肽的释放，同时递送该肽而不受环境干扰。以前的研究使用牛血清白蛋白（BSA）作为模型肽，研究从海藻酸盐微胶囊的释放特性。BSA的大小为66 kDa，而此处感兴趣的肽，连接蛋白-43羧基末端模拟肽（α CT 1），仅为3.4 kDa。这种大小的变化导致一组非常不同的释放参数。我们的总体目标是持续释放超过24小时。将肽长期应用于伤口部位以研究治疗效果是理想的。因此，已经探索了使用海藻酸盐微胶囊并沿着添加聚-L-赖氨酸和聚-L-鸟氨酸的扩散方法。我们首先旨在通过一组参数测试来建立和表征我们的参数。聚合物包衣的变化、pH的变化和负载比的变化先前已显示使用模型化合物影响释放。在这里，我们测试这些参数的特定变化，以显示对α CT 1释放的影响。此外，通过紫外线交联将微囊附着到几种生物材料和外科植入物上，以研究附着和递送的有效性。使用相差显微镜、扫描电子显微镜和原子力显微镜进行分析和测量，以表征微胶囊形态的变化。
A need exists to prolong the release of rapidly metabolized peptides of a low molecular weight, while delivering this peptide without environmental interference. Previous studies have used bovine serum albumin (BSA) as a model peptide to study release characteristics from alginate microcapsules. BSA is 66 kDa in size, while the peptide of interest here, connexin-43 carboxyl-terminus mimetic peptide (alpha CT1), is only 3.4 kDa. Such a change in size results in a much different set of release parameters. Our overall goal is a sustained release over a 24+ h period. Prolonged application of the peptide to a wound site to investigate therapeutic effects is ideal. As a result, a diffusion method using alginate microcapsules, along with the addition of poly-l-lysine and poly-l-ornithine, has been explored. We first aimed to establish and characterize our parameters through a set of parametric tests. Variations in polymer coating, change in pH, and changes in loading ratio have previously been shown to effect release using model compounds. Here we test specific changes in these parameters to show effects on the release of alpha CT1. Additionally, the microcapsules were attached to several biomaterials and surgical implants by ultraviolet cross-linking to study the effectiveness of attachment and delivery. Analysis and measurements using phase contrast microscopy, scanning electron microscopy, and atomic force microscopy were used to characterize changes in microcapsule morphology.