Distribution of putative denitrifying methane oxidizing bacteria in sediment of a freshwater lake, Lake Biwa
Distribution of putative denitrifying methane oxidizing bacteria in sediment of a freshwater lake, Lake Biwa
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DOI：
10.1016/j.syapm.2012.03.005
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发表时间：
2012-06-01
影响因子：
3.4
通讯作者：
Fukui, Manabu
中科院分区：
文献类型：
--
作者：
Kojima, Hisaya;Tsutsumi, Masazumi;Fukui, Manabu
甲烷氧化耦合反硝化是由隶属于候选门NC10的‘Candidatus methylirabilis oxyfera’介导的。研究了日本琵瓦湖淡水湖泊中疑似与“M. oxyfera”有关的反硝化甲烷氧化细菌的分布。在深层沉积物的表层中，对16S rRNA基因进行PCR分析，在NC10细菌中最常检测到与“M. oxyfera”密切相关的种型。在沉积物中，在pmoA基因库中也检测到与“M. oxyfera”相关的序列。通过催化报告细胞沉积荧光原位杂交（CARD-FISH）证实了NC10细菌的存在。变性梯度凝胶电泳（DGGE）和实时荧光定量PCR显示，深层沉积物上层与“m.o oxfera”相关的种型丰度较高。利用DGGE对湖泊沉积物中甲烷氧化菌的水平分布进行了分析，结果表明其主要分布在深水区。这些结果与前人对另一个淡水湖的研究结果一致，表明深层沉积物上层是反硝化甲烷氧化菌的主要栖息地。(c) 2012 Elsevier GmbH版权所有。
Methane oxidation coupled to denitrification is mediated by 'Candidatus methylomirabilis oxyfera', which belongs to the candidate phylum NC10. The distribution of putative denitrifying methane-oxidizing bacteria related to "M. oxyfera" was investigated in a freshwater lake, Lake Biwa, Japan. In the surface layer of the sediment from a profundal site, a phylotype closely related to "M. oxyfera" was most frequently detected among NC10 bacteria in PCR analysis of the 16S rRNA gene. In the sediment, sequences related to "M. oxyfera" were also detected in a pmoA gene library. The presence of NC10 bacteria was also confirmed by catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH). Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR indicated that the abundance of the "M. oxyfera"-related phylotype was higher in the upper layers of the profundal sediment. The horizontal distribution of the putative methanotrophs in lake sediment was also analyzed by DGGE, which revealed that their occurrence was restricted to deep water areas. These results agreed with those in a previous study of another freshwater lake, and suggested that the upper layer of the profundal sediments is the main habitat for denitrifying methanotrophs. (c) 2012 Elsevier GmbH. All rights reserved.