Relationship between total homocysteine and folate levels in pregnant women and their newborn babies according to maternal serum levels of vitamin B12
Relationship between total homocysteine and folate levels in pregnant women and their newborn babies according to maternal serum levels of vitamin B12
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DOI：
10.1016/s1470-0328(02)01307-1
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发表时间：
2002-07-01
影响因子：
5.8
通讯作者：
D'Almeida, V
中科院分区：
文献类型：
--
作者：
Guerra-Shinohara, EM;Paiva, AA;D'Almeida, V
目的根据孕妇维生素B-12（B12）水平测定孕妇血清中总同型半胱氨酸和叶酸水平，分析孕妇B12水平对其新生儿营养状况的影响。巴西。样本六十九名孕妇和他们各自的新生儿在deliver.Methods分娩前8小时收集产妇的血液。在胎盘排出后收集脐带血。采用高效液相色谱法测定总同型半胱氨酸，采用离子捕获法测定叶酸，采用酶免疫法测定B-12。主要观察指标孕妇和新生儿低维生素B-12水平与总同型半胱氨酸和叶酸水平的关系。但在母体B-12和新生儿红细胞（RBC）叶酸之间没有。母亲B-12水平与新生儿血清叶酸水平之间存在弱相关性。然而，母亲和新生儿总同型半胱氨酸水平之间以及新生儿B-12和新生儿总同型半胱氨酸水平之间存在显著相关性。平均（+/-SD）脐带血B-12、RBC叶酸、血清叶酸和总同型半胱氨酸水平分别为母体B-12、RBC叶酸、血清叶酸和总同型半胱氨酸值的1.7 +/-0.8、1.8 +/- 0.8、2.2 +/- 0.8和0.9 +/- 0.3倍，结论低B-12水平的孕妇不能为胎儿提供所需的B-12。临床后果可能是存在神经系统异常以及缺乏利用高半胱氨酸进行甲硫氨酸转化。
Objective To determine total homocysteine and folate levels in pregnant women according to vitamin B-12 (B-12) levels, and to analyse the impact of maternal B-12 levels on the nutritional status of their newborn babies.Design Cross sectional observational study.Setting Two public hospitals in Jundiai City, Sao Paulo, Brazil.Sample Sixty-nine pregnant women and their respective newborn babies at the time of delivery.Methods Maternal blood was collected up to 8 hours before delivery. Umbilical cord blood was collected after the expulsion of the placenta. Total homocysteine was measured by high performance liquid chromatography, folate by ion capture methodology and B-12 by enzyme immunoassay.Main outcome measures Relationship between low maternal vitamin B-12 levels and total homocysteine and folate levels in pregnant women and newborn babies.Results There was a significant correlation between maternal and neonatal B-12 levels, but not between maternal B-12 and neonatal red blood cell (RBC) folate. There was a weak correlation between maternal B-12 levels and neonatal serum folate. However, there were significant correlations between maternal and neonatal total homocysteine levels and between neonatal B-12 and neonatal total homocysteine levels. Mean (+/-SD) umbilical cord blood B-12, RBC folate, serum folate and total homocysteine levels were 1.7 +/- 0.8, 1.8 +/- 0.8, 2.2 +/- 0.8 and 0.9 +/- 0.3 times higher than maternal B-12, RBC folate, serum folate and total homocysteine values, respectively.Conclusions These data suggest that pregnant women with low B-12 levels are unable to provide the necessary amount of B-12 to their fetuses. The clinical consequences could be the presence of neurological abnormalities as well as the lack of utilisation of homocysteine for methionine transformation.