Dendrimers with tetrabenzoporphyrin cores: near infrared phosphors for in vivo oxygen imaging
Dendrimers with tetrabenzoporphyrin cores: near infrared phosphors for in vivo oxygen imaging
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DOI：
10.1016/s0040-4020(03)00432-0
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发表时间：
2003-05-26
期刊：
影响因子：
2.1
通讯作者：
Vinogradov, SA
中科院分区：
文献类型：
--
作者：
Rietveld, IB;Kim, E;Vinogradov, SA
四苯并卟啉 (PdTBP) 的钯配合物是强近红外磷光体，适合通过磷光猝灭进行组织氧成像。对于体内使用，PdTBP 荧光粉必须是水溶性的，并防止与血液中的生物大分子相互作用。提出了一种基于树枝状封装的生物相容性 PdTBP 荧光粉的构建方法。合成了一系列聚谷氨酸PdTBP树枝状聚合物，并研究了它们的光学和猝灭性能。同时保留了碱性 PdTBP 的优异光学特性（lambda(max) (Q)=630 nm，phi(phos)=12%），并且具有高度水溶性。 PdTBP-树枝状聚合物仍然表现出过高的氧猝灭常数(2000-3000 mmHg(-1)s(-1))并与亲脂性蛋白质(例如血清白蛋白)形成复合物。用聚乙二醇链对树枝状聚合物外围进行修饰会在一定程度上降低猝灭常数，但不会阻止磷光体与白蛋白的结合。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
Pd complexes of tetrabenzoporphyrins (PdTBPs) are strong near infra-red phosphors, suitable for tissue oxygen imaging by phosphorescence quenching. For in vivo use, PdTBP phosphors have to be water-soluble and protected from interactions with biomacromolecules in the blood. An approach to the construction of biologically compatible PdTBP-phosphors based on dendritic encapsulation is proposed. A series of polyglutamic PdTBP-dendrimers was synthesized and their optical and quenching properties were studied. While retaining the excellent optical characteristics (lambda(max) (Q)=630 nm, phi(phos)=12%) of basic PdTBPs and in addition being highly water soluble. PdTBP-dendrimers still exhibit excessively high oxygen quenching constants (2000-3000 mmHg(-1)s(-1)) and form complexes with lipophilic proteins, such as serum albumin. Modification of the dendrimer periphery with polyethyleneglycol chains somewhat reduces the quenching constants but does not prevent binding of the phosphors to albumin. (C) 2003 Elsevier Science Ltd. All rights reserved.