Decline of Lactate in Tumor Tissue After Ketogenic Diet: In Vivo Microdialysis Study in Patients with Head and Neck Cancer
Decline of Lactate in Tumor Tissue After Ketogenic Diet: In Vivo Microdialysis Study in Patients with Head and Neck Cancer
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DOI：
10.1080/01635581.2013.804579
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发表时间：
2013-08-01
影响因子：
2.9
通讯作者：
Wollenberg, B.
中科院分区：
文献类型：
--
作者：
Schroeder, U.;Himpe, B.;Wollenberg, B.
在头颈鳞状细胞癌（HNSCC）中，有氧糖酵解是肿瘤能量供应的关键特征。定量微透析 (D) 提供了一种在线测量体内碳水化合物代谢参数的方法。目的是标准化 HNSCC 患者的定量 D 研究，并证明生酮饮食是否会对肿瘤组织的碳水化合物代谢产生不同的影响。将市售的 100kDa-CMA71-D- 导管植入 HNSCC 患者的无肿瘤组织和肿瘤组织中，进行长达 5 天的同步测量。在西方饮食的 24 小时和随后长达 4 天的生酮饮食期间，监测尿素、葡萄糖、乳酸和丙酮酸的代谢模式和昼夜节律。生酮饮食3天后，肿瘤组织中的平均乳酸浓度比无肿瘤粘膜中的平均乳酸浓度下降幅度更大，而平均葡萄糖和丙酮酸浓度则上升。肿瘤组织的体内葡萄糖代谢明显受到营养的影响。生酮饮食后肿瘤组织中平均乳酸浓度的下降支持了 HNSCC 肿瘤细胞可能使用乳酸作为氧化葡萄糖代谢的燃料的假设。
In head and neck squamous cell carcinoma (HNSCC) aerobic glycolysis is the key feature for energy supply of the tumor. Quantitative microdialysis (D) offers an online method to measure parameters of the carbohydrate metabolism in vivo. The aim was to standardize a quantitative D-study in patients with HNSCC and to prove if a ketogenic diet would differently influence the carbohydrate metabolism of the tumor tissue. Commercially available 100kDa-CMA71-D- catheters were implanted in tumor-free and in tumor tissue in patients with HNSCC for simultaneous measurements up to 5days. The metabolic pattern and circadian rhythm of urea, glucose, lactate, and pyruvate was monitored during 24h of western diet and subsequent up to 4days of ketogenic diet. After 3days of ketogenic diet the mean lactate concentration declines to a greater extent in the tumor tissue than in the tumor-free mucosa, whereas the mean glucose and pyruvate concentrations rise. The in vivo glucose metabolism of the tumor tissue is clearly influenced by nutrition. The decline of mean lactate concentration in the tumor tissue after ketogenic diet supports the hypothesis that HNSCC tumor cells might use lactate as fuel for oxidative glucose metabolism.