Synergistic antimicrobial activity based on the combined use of a gemini-quaternary ammonium compound and ultraviolet A light
Synergistic antimicrobial activity based on the combined use of a gemini-quaternary ammonium compound and ultraviolet A light
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基于双子季铵化合物和紫外线 A 光联合使用的协同抗菌活性
DOI：
10.1016/j.jphotobiol.2013.11.027
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Hideaki Maseda and Takeshi Omasa
中科院分区：
文献类型：
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作者：
Akihiro Shirai;Mutsumi Aihara;Akira Takahashi;Hideaki Maseda and Takeshi Omasa
本研究考察了双季铵盐（即双季铵盐，即3,3 ' -（2,7-二氧杂辛烷）双（1-癸基溴化吡啶））作为有机杀生剂与峰值波长为365 nm的紫外- a （UV-A）发光二极管（LED）照射联合进行协同消毒的效用。该组合系统代表了一种新的消毒方法，利用便利的就地氧化，依赖于由gemini-QUAT在细菌膜上的初始作用引发的活性氧（ROS）的过量生产。我们证明，这种组合以显著和快速的方式降低了致病菌的活力，并且取决于gemini-QUAT的剂量和影响：联合程序使大肠杆菌的活力降低了5.0 log以上，但在没有gemini-QUAT的情况下，在相同的影响剂量下暴露于紫外线，其活力下降仅为2.3 log。添加过氧化氢酶作为自由基清除剂，通过联合消毒程序降低了细菌的失活。流式细胞术分析表明，使用联合消毒程序处理的细胞内超氧化物和过氧化氢过量产生。过量的超氧化物，仅在联合系统中检测到，似乎是由gemini-QUAT在细菌膜上的作用产生的，导致系统中ROS的过量和快速生成。我们的数据强烈表明，在初始联合处理时，这种ROS促进了细菌膜的过氧化，导致DNA的氧化修饰增加。这些氧化反应可能在这种消毒程序的效果中起重要作用。
This study examined the utility of synergistic disinfection employing a gemini-quaternary ammonium salt (a gemini-QUAT, namely 3,3′-(2,7-dioxaoctane)bis(1-decylpyridinium bromide)), as an organic biocide in combination with irradiation by an ultraviolet-A (UV-A) light-emitting diode (LED) with a peak wavelength of 365 nm. The combined system represents a novel disinfection method utilizing facilitatedin situoxidation depending on overproduction of reactive oxygen species (ROS) triggered by the initial action of the gemini-QUAT on the bacterial membrane. We demonstrate that this combination decreased the viability of pathogenic bacteria in a significant and rapid manner, and depended on doses of the gemini-QUAT and the fluence: the viability ofEscherichia coliwas reduced by greater than 5.0-logs by the combination procedure, but the decrease in viability was only 2.3-logs for exposure to UV at the same fluence dose in the absence of the gemini-QUAT. Adding catalase as a radical scavenger decreased bacterial inactivation by the combined disinfection procedure. Flow cytometric analysis indicated superoxide and hydrogen peroxide overproduction within cells treated with the combined disinfection procedure. The excessive superoxide, detected only in the combined system, appeared to be generated by the action of the gemini-QUAT at the bacterial membrane, leading to excessive and rapid generation of ROS in the system. Our data strongly suggested that this ROS promoted bacterial membrane peroxidation during initial treatment by the combination method, resulting in increased oxidative modification of DNA. These oxidative reactions may play an important role in the efficacy of this disinfection procedure.