Development of smartphone-based ECL sensor for dopamine detection: Practical approaches
Development of smartphone-based ECL sensor for dopamine detection: Practical approaches
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DOI：
10.1016/j.rechem.2020.100029
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发表时间：
2020-01-01
影响因子：
2.3
通讯作者：
Uppala, Padma P. Tadi
中科院分区：
文献类型：
--
作者：
Kwon, Hyun J.;Rivera, Elmer Ccopa;Uppala, Padma P. Tadi
在这项工作中，利用手机摄像头、丝网印刷电极(SPE)和移动APP开发了一种紧凑的、基于手机的ECL传感器装置，用于多巴胺的检测。从手机本身和外部电源两个方面对ECL反应的直流电压施加方法进行了全面的研究。在优化的传感条件下，以一次性碳固相萃取和20 mM三丙胺(TPrA)为辅料，批内和批间RSD分别为6.7%和5.5%，重现性和重现性均可接受。生化化合物多巴胺因其ECL猝灭特性和临床意义而被检测。根据Stern-Volmer方程常数的估算，研究了Ru(Bpy)(3)(2+)/TPrA被多巴胺猝灭的机理。多巴胺的线性范围为1.0~50µM(R2=0.982)。由于开发的基于手机的ECL传感器简单、小巧、由低成本元件组装而成，它为开发廉价的分析方法和紧凑型传感器提供了新的机会。(C)2020年提交人(S)。爱思唯尔出版公司(Elsevier B.V.)
In this work, a compact, mobile phone-based ECL sensor apparatus was developed using the phone cameras, screen-printed electrodes (SPE), and mobile app for dopamine detection. Methods of DC voltage application for ECL reaction were comprehensively studied from the mobile phone itself or external power. Under optimized sensing conditions, with disposable carbon SPE and 20 mM coreactant tri-n-propylamine (TPrA), acceptable re-peatability and reproducibility were achieved in terms of relative standard deviation (RSD) of intra- and interassays, which were 6.7 and 5.5%, respectively. The biochemical compound dopamine was measured due to its ECL quenching characteristics and its clinical importance. The quenching mechanism of Ru(bpy)(3)(2+)/TPrA by dopamine was investigated based on the estimation of the constants of the Stern-Volmer equations. The linear range for detectable dopamine concentration was from 1.0 to 50 mu M (R-2 = 0.982). As the developed mobile phone-based ECL sensor is simple, small and assembled from low-cost components, it offers new opportunities for the development of inexpensive analytical methods and compact sensors. (C) 2020 The Author(s). Published by Elsevier B.V.