Complement proteins are present in developing endochondral bone and may mediate cartilage cell death and vascularization
Complement proteins are present in developing endochondral bone and may mediate cartilage cell death and vascularization
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DOI：
10.1006/excr.1996.0269
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发表时间：
1996-09-15
影响因子：
3.7
通讯作者：
Sorgente, N
中科院分区：
文献类型：
--
作者：
Andrades, JA;Nimni, ME;Sorgente, N
正常的软骨内骨形成遵循时间顺序：未成熟或静止的软骨细胞从静止区移动，增殖，变平，排列成柱状，最后变得肥大，解体，并被骨取代。指导这一过程的机制还不完全清楚，但它们包括程序性细胞死亡，这是发育和一些疾病过程中的重要阶段。我们用免疫荧光法研究了各种补体蛋白的分布，以检验这一系列事件，特别是细胞崩解和基质溶解是补体介导的这一假设。这些研究的结果表明，补体蛋白C3和因子B均匀地分布在静止区和增殖区。备解素定位于静止区和肥大区，但不在增殖区。补体蛋白C5和C9仅位于肥大区。这种解剖学上分离的分布模式表明，补体蛋白可能是重要的软骨-骨转化和替代途径参与。(C)出版社：Academic Press，Inc.
Normal endochondral bone formation follows a temporal sequence: immature or resting chondrocytes move away from the resting zone, proliferate, flatten, become arranged into columns, and finally become hypertrophic, disintegrate, and are replaced by bone. The mechanisms that guide this process are incompletely understood, but they include programmed cell death, a stage important in development and some disease processes. Using immunofluorescence we have studied the distribution of various complement proteins to examine the hypothesis that this sequence of events, particularly cell disintegration and matrix dissolution, are complement mediated, The results of these studies show that complement proteins C3 and Factor B are distributed uniformly in the resting and proliferating zones. Properdin is localized in the resting and hypertrophic zone but not in the proliferating zone. Complement proteins C5 and C9 are localized exclusively in the hypertrophic zones. This anatomically segregated pattern of distribution suggests that complement proteins may be important in cartilage-bone transformation and that the alternate pathway is involved. (C) 1996 Academic Press, Inc.