Effects of colchicine on cardiac cell function indicate possible role for membrane surface tubulin.
Effects of colchicine on cardiac cell function indicate possible role for membrane surface tubulin.
复制标题
秋水仙碱对心脏细胞功能的影响表明膜表面微管蛋白可能发挥作用。
DOI：
10.1016/0014-4827(86)90044-3
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发表时间：
1986
影响因子：
3.7
通讯作者：
Rubin,RW
中科院分区：
文献类型：
--
作者：
Lampidis,TJ;Trevorrow,KW;Rubin,RW
研究了微管蛋白结合药物秋水仙碱对培养的新生心肌细胞功能的影响。应用低剂量的秋水仙碱（但不是lumicolchicine）引起早期可逆的增加，在跳动率伴随着幅度下降。用一定剂量的胰蛋白酶处理细胞，去除表面微管蛋白，但不抑制自发搏动，减弱了秋水仙素的作用。表面放射性碘标记的活培养物，然后进行二维凝胶电泳和放射自显影显示，两个斑点被大量标记。这些斑点与纯化的脑微管蛋白共迁移。来自心脏培养物的成纤维细胞没有标记在微管蛋白斑点上。胰蛋白酶处理从放射自显影图中去除了假定的微管蛋白，但仅从染色凝胶中去除了α-微管蛋白斑点的最基本部分。这些结果与新生儿心肌细胞中微管蛋白同种型的表面膜作用一致。
The effects of the tubulin-binding drug colchicine on cultured neonate cardiac cell function were investigated. Application of low doses of colchicine (but not lumicolchicine) caused an early reversible increase in beating rate with a concomitant decrease in amplitude. Treatment of the cells with trypsin at a dose that removes surface tubulin but does not inhibit spontaneous beating, diminished the colchicine effect. Surface radio-iodination of the live cultures followed by two-dimensional gel electrophoresis and radioautography revealed that two spots were heavily labeled. These spots co-migrated with purified brain tubulin. Fibroblasts derived from the cardiac cultures did not label over the tubulin spots. Trypsin treatment removed the presumptive tubulin from the radioautographs but only removed the most basic portion of the α-tubulin spot from the stained gel. These results are consistent with a surface membrane role for an iso-form of tubulin in neonate cardiac cells.