Replication of Genetic Information with Self-Encoded Replicase in Liposomes
Replication of Genetic Information with Self-Encoded Replicase in Liposomes
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DOI：
10.1002/cbic.200800360
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发表时间：
2008-10-13
期刊：
影响因子：
3.2
通讯作者：
Yomo, Tetsuya
中科院分区：
文献类型：
--
作者：
Kita, Hiroshi;Matsuura, Tomoaki;Yomo, Tetsuya
在所有有生命的系统中，基因组都是由基因组本身编码的蛋白质复制的。这种普遍的反应对于该体系的发展至关重要。在这里，我们构建了一个简化的系统，它涉及被包裹的大分子，被称为“自编码系统”，在这个系统中，遗传信息通过脂质体中的自编码复制酶来复制。也就是说，普遍的反应是在一个被脂类双层结合的微格内重新组成的。该系统以一个模板RNA序列作为信息分子，以纯化的翻译因子重组的体外翻译系统作为信息解码的机制。在这个系统中，Qβ复制酶的催化亚单位是从编码蛋白质的模板RNA合成的。然后，复制酶复制用于其生产的模板RNA。因此，脂质体内自编码系统是现有的最简单的此类系统之一；它只由144个基因产物组成，而其复制的信息和功能被编码在不同的分子上，并被划分到微环境中以便于进化。
In all living systems, the genome is replicated by proteins that are encoded within the genome itself. This universal reaction is essential to allow the system to evolve. Here, we have constructed a simplified system involving encapsulated macromolecules termed a "self-encoding system", in which the genetic information is replicated by self-encoded replicase in liposomes. That is the universal reaction was reconstituted within a microcompartment bound by a lipid bilayer. The system was assembled by using one template RNA sequence as the information molecule and an in vitro translation system reconstituted from purified translation factors as the machinery for decoding the information. In this system, the catalytic subunit of Q beta replicase is synthesized from the template RNA that encodes the protein. The replicase then replicates the template RNA that was used for its production. Thus in-liposome self-encoding system is one of the simplest such systems available; it consists of only 144 gene products, while the information and the function for its replication are encoded on different molecules and are compartmentalized into the microenvironment for evolvability.