C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation.
C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation.
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C6 神经胶质瘤细胞产生碱性成纤维细胞生长因子，可以刺激自身增殖。
DOI：
10.1093/oxfordjournals.jbchem.a122950
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发表时间：
1989
影响因子：
2.7
通讯作者：
H. Nakagawa
中科院分区：
文献类型：
--
作者：
N. Okumura;K. Takimoto;M. Okada;H. Nakagawa
通过纯化碱性成纤维细胞生长因子（bFGF），研究其对C6胶质瘤细胞的促生长作用。用其抗体检测培养的胶质瘤细胞是否能产生它。结果表明，在无血清培养基中，bFGF能促进C6胶质瘤细胞的DNA合成和增殖，且在肝素的作用下，bFGF对C6胶质瘤细胞的半最大刺激浓度分别为0.2和5 ng/ml。肝素的这种作用是剂量依赖性的，在0.5微克/毫升时达到一半最大值。下一步，我们提高抗bFGF血清，并通过免疫印迹分析检测C6胶质瘤细胞提取物的单一免疫反应带。免疫反应物质在肝素- sepharose柱上部分纯化，并被证明可以刺激C6胶质瘤细胞的DNA合成。根据其免疫反应性、分子量、对肝素的亲和力和促生长活性，确定该物质为bFGF。细胞中bFGF含量随细胞密度的增加而升高，但在细胞的条件培养基中未检测到免疫反应性。这些结果表明，C6胶质瘤细胞产生并储存bFGF，这对刺激其自身的生长是有效的。
With purified preparations of basic fibroblast growth factor (bFGF), we studied the effect of its growth-promoting activity on C6 glioma cells. We also examined with its antibody whether the cultured glioma cells could produce it. It was shown that bFGF stimulated the DNA synthesis and proliferation of C6 glioma cells in serum-free medium, and that the activity was potentiated by heparin, the bFGF concentrations for half-maximal stimulation being 0.2 and 5 ng/ml in the presence and absence of heparin, respectively. This effect of heparin was dose-dependent and was half-maximal at 0.5 microgram/ml. Next, we raised the antiserum against bFGF and detected a single immunoreactive band from extracts of C6 glioma cells by immunoblot analysis. The immunoreactive substance was partially purified on a heparin-Sepharose column and was shown to stimulate the DNA synthesis of C6 glioma cells. On the basis of its immunoreactivity, molecular weight, affinity for heparin, and growth-promoting activity, this substance was identified as bFGF. The content of bFGF in the cells was elevated as the cell density increased, but no immunoreactivity was detected in the conditioned medium of the cells. These results suggest that C6 glioma cells produce and store bFGF which is potent in stimulating their own growth.