Effect of a prostaglandin I2 analog on the expression of thrombomodulin in liver and spleen endothelial cells after an extensive hepatectomy
Effect of a prostaglandin I2 analog on the expression of thrombomodulin in liver and spleen endothelial cells after an extensive hepatectomy
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前列腺素I2类似物对广泛肝切除术后肝和脾内皮细胞血栓调节蛋白表达的影响
DOI：
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
S. Isaji
中科院分区：
文献类型：
--
作者：
M. Usui;H. Kato;N. Kuriyama;Y. Azumi;M. Kishiwada;S. Mizuno;H. Sakurai;M. Tabata;Tatsuya Hayashi;Koji Suzuki;S. Isaji
目的肝切除术后残肝功能障碍是由于内皮细胞(EC)损伤形成微血栓所致。采用体外培养的人脐静脉内皮细胞(HUVECs)和犬大范围肝切除模型，观察前列腺素I2(PGI2)对血管内皮细胞抗凝活性标志物血栓调节蛋白(TM)表达的影响。方法采用逆转录聚合酶链式反应(RT-PCR)检测HUVECs表面是否存在PGI2受体，并用酶联免疫吸附试验检测PGI2类似物对HUVECs血栓调节蛋白(TM)表达的影响。20只杂种犬随机分为假手术组、70%肝切除组、84%肝切除组、84%肝切除组和84%肝切除组，分别给予PGI2类似物，用免疫组织化学方法检测肝、脾、胰腺、肾、肺、门静脉、肠组织中TM的表达。84%肝切除后，肝窦和脾静脉窦内皮细胞上TM的表达明显减少，而84%肝切除加PGI2类似物后，这种损伤明显减轻。结论广泛肝切除不仅对肝窦和脾静脉窦内皮细胞造成严重的损伤，而且对脾静脉窦内皮细胞也造成严重的损伤。前列腺素I2阻止了对这些内皮细胞的损伤，这表明PGI2改善了残余肝脏的微循环。
PurposeDysfunction of the remnant liver after a hepatectomy is caused by microthrombus formation due to endothelial cell (EC) damage. This study evaluated the effect of prostaglandin I2 (PGI2) on the expression of thrombomodulin (TM), a marker for the anticoagulant properties of ECs, using cultured human umbilical vein endothelial cells (HUVECs), and using a canine extensive hepatectomy model.MethodsThe presence of PGI2 receptors was confirmed on HUVECs by reverse transcription-polymerase chain reaction, and the effect of the PGI2 analog on TM expression on HUVECs was determined by an enzyme-linked immunosorbent assay. Twenty mongrel dogs were divided into four groups comprising a sham operation, 70% hepatectomy, 84% hepatectomy, and 84% hepatectomy, with the administration of the PGI2 analog, respectively, and TM expression in the liver, spleen, pancreas, kidney, lung, portal vein, and intestine was determined immunohistochemically.ResultsThe TM expression on HUVECs was upregulated by the PGI2 analog. The TM expression on ECs in the hepatic sinusoids and splenic sinus were markedly decreased after the 84% hepatectomy, but such damage was markedly mitigated following an 84% hepatectomy with administration of the PGI2 analog.ConclusionsAn extensive hepatectomy induced severe EC damage not only in the hepatic sinusoids but in the splenic sinuses as well. Prostaglandin I2 prevented damage to these ECs, suggesting that PGI2 improves the microcirculation in the remnant liver.
DOI：
10.1073/pnas.83.10.3460
发表时间：
1986-05-01
影响因子：
11.1
作者：
NAWROTH, PP;HANDLEY, DA;STERN, DM
通讯作者：
STERN, DM
DOI：
10.1016/s0021-9258(19)68276-1
发表时间：
1982-01
期刊：
The Journal of biological chemistry
影响因子：
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作者：
N. Esmon;W. Owen;C. Esmon
通讯作者：
N. Esmon;W. Owen;C. Esmon
DOI：
10.1016/s0021-9258(17)44163-9
发表时间：
1983-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
N. Esmon;R. Carroll;C. Esmon
通讯作者：
N. Esmon;R. Carroll;C. Esmon