MOLECULAR EVIDENCE OF FETAL-DERIVED CHROMOSOME-21 MARKERS (STRS) IN TRANSCERVICAL SAMPLES
MOLECULAR EVIDENCE OF FETAL-DERIVED CHROMOSOME-21 MARKERS (STRS) IN TRANSCERVICAL SAMPLES
复制标题
DOI：
10.1002/pd.1970150108
复制
发表时间：
1995-01-01
影响因子：
3
通讯作者：
RODECK, C
中科院分区：
文献类型：
--
作者：
ADINOLFI, M;SHERLOCK, J;RODECK, C
在妊娠 8-13 周时通过冲洗或抽吸收集的宫颈细胞 (TCC)，用于分析是否存在胎儿来源的 DNA 序列。通过聚合酶链式反应 (PCR) 扩增从母体外周血、TCC 样本和胎盘组织中提取的 DNA，以检测 21 号染色体特异的小串联重复 (STR) 标记。在 4 个 TCC 样本中可以清楚地观察到胎儿来源的 STR 产物。通过冲洗或抽吸收集的 TCC 样品还同时使用 X 和 Y 探针通过荧光原位杂交 (FISH) 进行分析：在从具有男性胎儿的母亲获得的所有 TCC 样品中均可检测到 46,XY 细胞。
Transcervical cells (TCCs), collected by flushing or aspiration at 8-13 weeks of gestation, were analysed for the presence of fetal-derived DNA sequences. DNA extracted from maternal peripheral blood, TCC samples, and placental tissue was amplified by the polymerase chain reaction (PCR) to detect small tandem repeat (STR) markers specific to chromosome 21. STR products of fetal origin could be clearly observed in four TCC samples. TCC samples collected by flushing or aspiration were also analysed by fluorescent in situ hybridization (FISH) using X and Y probes simultaneously: 46,XY cells could be detected in all TCC samples obtained from mothers with male fetuses.