Functional recovery and histomorphometric assessment following tibial nerve injury in the mouse
Functional recovery and histomorphometric assessment following tibial nerve injury in the mouse
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DOI：
10.1055/s-2003-37190
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发表时间：
2003-01-01
影响因子：
2.1
通讯作者：
Mackinnon, SE
中科院分区：
文献类型：
--
作者：
George, LT;Myckatyn, TM;Mackinnon, SE
纵向研究已经确定，可以在小鼠中评估坐骨神经损伤后的功能恢复。胫神经损伤提供了坐骨神经损伤的几个优点，包括改善下肢感觉和终末器官神经再支配。在55只C3 H小鼠中研究了胫神经挤压损伤后的功能恢复，将其随机分为5组，从正常神经或术后2、3、4或6周收获用于组织形态学评价。术前和术后定期获得行走轨迹，并测量足迹长度。术后14天打印长度显著正常化，术后28天完全恢复。术后2 ~ 4周检测到明显的神经再生组织形态学证据。胫神经损伤是研究小鼠神经再生的坐骨神经的可行替代方法，并且在该模型中，印迹长度因子可用于监测功能恢复。
Longitudinal studies have established that functional recovery following sciatic nerve injury can be evaluated in the mouse. Injury to the tibial nerve offers several advantages to sciatic nerve injury, including improved lower extremity sensation and end-organ reinnervation. Functional recovery following tibial nerve crush injury was studied in 55 C3H mice randomized into five groups harvested for histomorphometric evaluation from either normal nerves or 2, 3, 4, or 6 weeks postoperatively. Walking tracks were obtained preoperatively, and at regular intervals postoperatively, and foot print lengths measured. Significant normalization of print length occurred 14 days postoperatively, and complete recovery was noted 28 days postoperatively. Significant histomorphologic evidence of neuroregeneration was detected between 2 and 4 weeks postoperatively. Injury to the tibial nerve is a viable alternative to the sciatic nerve for studying neural regeneration in mice, and the print length factor can be used to monitor functional recovery in this model.