A comparative study on the composition of chlorosomes (Chlorobium vesicles) and cytoplasmic membranes from Chloroflexus aurantiacus strain Ok-70-fl and Chlorobium limicola f. thiosulfatophilum strain 6230
A comparative study on the composition of chlorosomes (Chlorobium vesicles) and cytoplasmic membranes from Chloroflexus aurantiacus strain Ok-70-fl and Chlorobium limicola f. thiosulfatophilum strain 6230
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橙色绿屈菌 Ok-70-fl 菌株和 limicola f 绿菌叶绿体 (Chlorobium vesicles) 和细胞质膜组成的比较研究。
DOI：
10.1007/bf00407024
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发表时间：
2004
影响因子：
2.8
通讯作者：
K. Schmidt
中科院分区：
文献类型：
--
作者：
K. Schmidt
从橙色绿藻Ok-70-fl和利氏氯杆菌6230中分离得到高纯度的叶绿体组分和细胞膜。对它们的色素、磷脂、糖脂和细胞色素含量以及琥珀酸脱氢酶和NADH-氧化酶的比活性进行了比较分析。结果表明，不同分离组份的色素沉着和磷脂含量有一定差异。柔性氯藻的氯小体含有一种特殊的BChl a-络合物，其特征吸收峰位于约790 nm处。在氯溴的叶绿体光谱中未检测到该Bchl a-络合物。两种生物细胞质膜的近红外区光谱有很大差异：氯离子膜的Bchl a-络合物在806和868 nm处有峰，而氯铍的膜在710 nm处有一个单一的Bchl a峰。与氯铋的研究结果相反，柔氯藻的氯小体的磷脂含量至少是细胞质膜的两倍。在绿体中，细胞质膜的磷脂含量是其绿体的三倍。所有其他成分(类胡萝卜素组成、酶活性、细胞色素含量和糖脂)在两个菌株中的分布大致相同。结果表明，光合作用结构的差异主要是由于细胞质膜上光合作用单位的组织不同以及叶绿体的受光系统与细胞膜上的反应中心的联系方式的不同。
Highly purified fractions of chlorosomes and cytoplasmic membranes were isolated fromChloroflexus aurantiacusOk-70-fl andChlorobium limicola6230. These fractions were comparatively analyzed for their pigmentation, phospholipid, glycolipid, and cytochromeccontent as well as for their specific activities of succinate dehydrogenase and NADH-oxidase. The data showed that there are some differences in pigmentation and phospholipid content between the isolated fractions ofChloroflexusandChlorobium. Chlorosomes ofChloroflexuscontained a specific BChl a-complex with a characteristic absorption maximum at about 790 nm. This BChl a-complex could not be detected in spectra of chlorosomes fromChlorobium. The near infrared region of the spectra of the isolated cytoplasmic membranes of both organisms revealed considerable differences: The BChl a-complexes ofChloroflexusmembranes exhibited peaks at 806 and 868 nm whereas the membranes ofChlorobiumhad a single BChl a-peak at 710 nm. In contrast to the findings withChlorobiumthe chlorosomes ofChloroflexuscontained at least twice as much phospholipids as did the cytoplasmic membranes. InChlorobiumthe phospholipid content of cytoplasmic membranes is three times that of their chlorosomes. The distribution of all other components (carotenoid composition, enzyme activities, cytochromeccontent, and glycolipids) was about the same in both strains. From the data it was concluded that differences in the organization of the photosynthetic apparatus are mainly based on differences of the organization of the photosynthetic units in the cytoplasmic membrane and probably the kind of linkage of the light harvesting system in the chlorosomes with the reaction center in the cytoplasmic membranes.