SYNTHESIS OF 20-ALPHA-HYDROXYLATED STEROIDS BY OVARIES OF THE DAB (LIMANDA-LIMANDA)
SYNTHESIS OF 20-ALPHA-HYDROXYLATED STEROIDS BY OVARIES OF THE DAB (LIMANDA-LIMANDA)
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DOI：
10.1016/0016-6480(89)90041-5
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发表时间：
1989-10-01
影响因子：
2.7
通讯作者：
SCOTT, AP
中科院分区：
文献类型：
--
作者：
CANARIO, AVM;SCOTT, AP
将Dab（Limanda limanda）卵巢碎片与[4，7 - 3 H]双烯醇酮或17 α-双烯醇酮在体外孵育。羟基[1，2，6，7 - 3 H]孕酮，以研究类固醇生成的模式。在dab卵巢中发现的主要酶是20 α。羟基类固醇脱氢酶在卵巢孵育物中初步鉴定的类固醇是17 α，20. α-二羟基-4-异戊烯-3-酮（17，20 α- P），17 α 20 α-二羟基-5 β-葡聚糖-3-酮，3 β，17. α，20. α-三羟基-5 β-十六烷（3 β，17，20. alpha. P-5 β），和3. α，17 α 20 α-三羟基-5 β- - 是的这些类固醇的存在下，血浆中的成熟女性和男性dabs进行了研究，通过放射免疫测定。产生抗17，20 α的抗血清。P.17，20.阿尔法。[3H]P通过用17 α-葡萄糖孵育欧鲽鱼精来产生标记。羟基[3 H]孕酮。放射免疫测定显示与5 β- 17，20 α-的还原类似物因此，与薄层色谱法结合使用，以测量类固醇。高浓度的17，20 α- P和3 β，17，20. alpha. P-5.beta.在雌性和雄性DAB血浆中发现。这些类固醇的可能作用进行了讨论。
Dab (Limanda limanda) ovarian fragments were incubated in vitro with either [4,7-3H]pregnenolone or 17.alpha.-hydroxy[1,2,6,7-3H]progesterone to investigate the pattern of steroidogenesis. A major enzyme found in the dab ovary was 20.alpha.-hydroxysteroid dehydrogenase. Among the steroids that were tentatively identified in ovarian incubates were 17.alpha.,20.alpha.-dihydroxy-4-pregnen-3-one (17,20.alpha.-P), 17.alpha.20.alpha.-dihydroxy-5.beta.-pregnan-3-one, 3.beta.,17.alpha.,20.alpha.-trihydroxy-5.beta.-pregnane (3.beta.,17,20.alpha.-P-5.beta.), and 3.alpha.,17.alpha.20.alpha.-trihydroxy-5.beta.-pregnane. The presence of these steroids in plasma of mature female and male dabs was studied by radioimmunoassay. The antiserum was raised against 17,20.alpha.-P. The 17,20.alpha.-[3H]P label was produced by incubating plaice milt with 17.alpha.-hydroxy [3H]progesterone. The radioimmunoassay was shown to have a high cross-reaction with the 5.beta.-reduced analogues of 17,20.alpha.-P and was therefore used, in conjunction with thin-layer chromatography, to measure the steroids. High concentrations of both 17,20.alpha.-P and 3.beta.,17,20.alpha.-P-5.beta. were found in female and male dab plasma. The possible role of these steroids is discussed.