NH2-terminal amino acid sequence of rabbit hepatopoietin A, a heparin-binding polypeptide growth factor for hepatocytes.
NH2-terminal amino acid sequence of rabbit hepatopoietin A, a heparin-binding polypeptide growth factor for hepatocytes.
复制标题
兔肝生成素 A 的 NH2 末端氨基酸序列，肝素结合多肽生长因子。
DOI：
10.1016/0006-291x(89)91130-3
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发表时间：
1989
影响因子：
3.1
通讯作者：
Michalopoulos,G
中科院分区：
文献类型：
--
作者：
Zarnegar,R;Muga,S;Enghild,J;Michalopoulos,G
肝素 A (HPTA) 是一种适用于肝细胞的酸性肝素结合多肽生长因子，其特性与其他已知的肝素结合生长因子不同。 HPTA是由重链和轻链组成的异二聚体，Mr分别为70,000和35,000。 HPTA 是肝细胞的完全有丝分裂原，因为它刺激维持在无血清培养基中的肝细胞中的 DNA 合成。我们在其他地方报道了它从兔血清或人血浆中的完全纯化（R. Zarnegar 和 G. Michalopoulos，1989）。在本通讯中，我们报告了 HPTA 轻链的 N 端氨基酸序列，最多 24 个残基 (VVNGKPTRTNVGRMVSLKYRNKHI)，并根据计算机搜索分析表明该序列是独特的，与任何其他蛋白质或生长因子无关。我们还制备了针对与 N 端氨基酸残基 1 至 24 序列相对应的合成肽的抗血清，该肽可识别整个 HPTA 分子。该抗血清以及对应于 HPTA N 端氨基酸序列的寡核苷酸探针可用作探针来鉴定该生长因子的起源组织并协助其基因的分子克隆。
Hepatopoietin A (HPTA) is an acidic heparin-binding polypeptide growth factor for hepatocytes with properties distinct from other known heparin-binding growth factors. HPTA is a heterodimer consisting of a heavy and a light polypeptide chain with Mr of 70,000 and 35,000 respectively. HPTA is a complete mitogen for hepatocytes in that it stimulates DNA synthesis in hepatocytes maintained in serum-free medium. Its complete purification from rabbit serum or human plasma was reported by us elsewhere (R. Zarnegar and G. Michalopoulos, 1989). In the present communication we report the N-terminal amino acid sequence of the HPTA light chain up to 24 residues (VVNGKPTRTNVGRMVSLKYRNKHI) and show that this sequence is unique and not related to any other proteins or growth factors based on computer search analysis. We have also raised antiserum against a synthetic peptide corresponding to the sequence of N-terminal amino acids residues 1 to 24. which recognizes the whole HPTA molecule. This antiserum as well as oligonucleotide probes corresponding to the N-terminal amino acid sequence of HPTA can be used as probes to identify tissue(s) of origin of this growth factor and assist in molecular cloning of its gene.