ALTERATIONS IN HYPOTHALAMIC AND PITUITARY-HORMONE LEVELS INDUCED BY NEONATAL TREATMENT OF FEMALE MICE WITH DIETHYLSTILBESTROL
ALTERATIONS IN HYPOTHALAMIC AND PITUITARY-HORMONE LEVELS INDUCED BY NEONATAL TREATMENT OF FEMALE MICE WITH DIETHYLSTILBESTROL
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DOI：
10.1016/0890-6238(92)90197-2
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发表时间：
1992-01-01
影响因子：
3.3
通讯作者：
HALLING, A
中科院分区：
文献类型：
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作者：
HALLING, A
NMRI品系的新生雌性小鼠用合成雌激素己烯雌酚（DES）处理，在出生后的前5天每天5 μ g，或仅用载体处理。用放射免疫分析法（RIA）测定6 ~ 56日龄雌兔垂体和血清LH、FSH及下丘脑LHRH水平，间隔6 ~ 7天。与对照组相比，DES处理的雌性动物在第6、12和21天的垂体中以及第6和12天的血清中LH水平较低;在第42天的垂体和血清中均观察到LH水平升高。第6天和第12天，DES给药雌性动物垂体和血清中的FSH水平降低;第21天，垂体中的FSH含量增加。在视前区和基底下丘脑的DES治疗的女性，LHRH水平增加，在第21天和第42天下降。在第56天，对照组和DES组的血清FSH和LH水平以及下丘脑LHRH含量相似。出生后第1至5天使用10（-2）μ g DES或更低剂量或5 μ g雌二醇-17-β（E2）治疗对血清LH没有抑制作用。下丘脑-垂体-卵巢反馈系统对15日龄对照组和DES处理组雌性动物卵巢切除术和E2激发的反应相似。DES治疗的56日龄女性卵巢切除术LH反应降低，但增加了外源性E2的反应相比，控制。这些结果表明，DES新生儿治疗对发育中的雌性小鼠的下丘脑-垂体系统具有显著影响，这可能对这些雌性小鼠成年后卵巢功能的改变具有重要意义。
Neonatal female mice of the NMRI strain were treated with the synthetic estrogen diethylstilbestrol (DES), 5-mu-g daily for the first 5 days after birth, or with vehicle only. Levels of LH and FSH (pituitary and serum) and LHRH (hypothalamus) were measured by radioimmunoassay (RIA) in 6- to 56-day-old females with 6- to 7-day intervals. Compared to controls, DES-treated females had low levels of LH on days 6, 12, and 21 in the pituitary, and on days 6 and 12 in serum; increased LH levels were seen in both the pituitary and serum on day 42. The FSH levels of DES-treated females were decreased on days 6 and 12 in the pituitary and on day 6 in serum; an increased FSH content occurred on day 21 in the pituitary. In the preoptic area and basal hypothalamus of DES-treated females, levels of LHRH were increased on day 21 and decreased on day 42. On day 56, the serum levels of FSH and LH and the hypothalamic content of LHRH were similar in controls and DES females.A second study including both synthetic and natural estrogen was performed in 12-day-old females. Treatment with 10(-2)-mu-g DES or lower doses or 5-mu-g estradiol-17-beta (E2) on days 1 to 5 after birth had no depressive effect on serum LH. The hypothalamic-pituitary-ovarian feedback system reacted similarly to ovariectomy and E2 challenge in 15-day-old control and DES-treated females. DES-treated 56-day-old females had a reduced LH response to ovariectomy but increased response to exogenous E2 compared to controls. These results show that neonatal treatment with DES has pronounced effects on the hypothalamic-pituitary system in the developing female mouse, which may be of importance for the altered ovarian function in these females as adults.