Quantification of D-amino acids in human urine using GC-MS and HPLC
Quantification of D-amino acids in human urine using GC-MS and HPLC
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使用 GC-MS 和 HPLC 定量人尿液中的 D-氨基酸
DOI：
10.1007/bf00805848
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发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
A. Friedrich
中科院分区：
文献类型：
--
作者：
H. Brückner;S. Haasmann;A. Friedrich
摘要使用手性相 (Chirasil-L-Val) 毛细管气相色谱法结合选择离子监测质谱法测定了 7 名健康志愿者（年龄 27 至 49 岁）尿液中游离 D-氨基酸 (D-AA) 的相对含量。游离D-AA的绝对量通过氨基酸与邻苯二甲醛和N-异丁酰-L-半胱氨酸的柱前衍生化，然后对形成的异吲哚衍生物进行高效液相色谱分离和荧光检测来测定。发现以下最丰富的 D-AA（绝对和相对量最高和最低）：D-Ser (379.8 — 30.1 µMol/L；56.5 — 19.0%)、D-Ala (53.8 — 7.6 µMol/L；19.6 — 5.7%)、D-Thr (5.8 — 0.25 µMol/L；3.4 — 1.0%)、D-Val (3.7 — 0μMol/L；4.2 — 0%) 和 D-Phe (3.5 — 0.35μMol/L；4.8 — 1.4%)。
SummaryThe relative amounts of free D-amino acids (D-AA) in the urine of seven healthy volunteers (age 27 to 49 years) were determined using chiral phase (Chirasil-L-Val) capillary gas chromatography in conjunction with selected ion monitoring mass spectrometry. The absolute amounts of free D-AA were determined by pre-column derivatization of the amino acids witho-phthaldialdehyde andN-isobutyryl-L-cysteine followed by high-performance liquid chromatographic separation and fluorescence detection of the isoindol derivatives formed. The following most abundant D-AA were found (highest and lowest absolute and relative amounts): D-Ser (379.8 — 30.1µMol/L; 56.5 — 19.0%), D-Ala (53.8 — 7.6µMol/L; 19.6 — 5.7%), D-Thr (5.8 — 0.25µMol/L; 3.4 — 1.0%), D-Val (3.7 — 0µMol/L; 4.2 — 0%), and D-Phe (3.5 — 0.35µMol/L; 4.8 — 1.4%).