Dynamics and diversity of bacteria associated with the disease vectors Aedes aegypti and Aedes albopictus
Dynamics and diversity of bacteria associated with the disease vectors Aedes aegypti and Aedes albopictus
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DOI：
10.1038/s41598-019-48414-8
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发表时间：
2019-08-21
影响因子：
4.6
通讯作者：
Loaiza, Jose R.
中科院分区：
文献类型：
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作者：
Bennett, Kelly L.;Gomez-Martinez, Carmelo;Loaiza, Jose R.
埃及伊蚊和白纹伊蚊在相同的水生场所发育，在那里它们遇到影响其生活史和传播人类虫媒病毒能力的微生物。一些细菌，如沃尔巴克氏体，目前正在考虑用于控制登革热，基孔肯雅和寨卡病毒。然而，人们对与伊蚊相关的细菌的动态和多样性知之甚少，包括塑造其时空分布的幼虫栖息地特征。我们应用大规模的16S rRNA扩增测序960成人和幼虫的Ae。埃及伊蚊和埃及伊蚊。白纹伊蚊从59个采样点广泛分布在巴拿马9个省。我们发现这两个物种共享一个有限的，但高度可变的核心微生物群，反映了其产卵栖息地的高随机性。尽管共享很大比例的微生物群，Ae。埃及伊蚊比埃及伊蚊具有更高的细菌多样性。白纹伊蚊，主要是由于在幼虫阶段的稀有细菌群。我们发现幼虫和成年蚊子的细菌群落之间存在显着差异，金属和陶瓷容器的样品之间也存在显着差异。然而，我们发现很少支持地理，水温和pH值作为细菌协会的预测。我们报告一个低发病率的自然沃尔巴克氏体感染的伊蚊和它的地理分布。这一基线信息为研究与伊蚊有关的细菌的功能和相互作用以及对巴拿马境内生物防治的影响奠定了基础。
Aedes aegypti and Aedes albopictus develop in the same aquatic sites where they encounter microorganisms that influence their life history and capacity to transmit human arboviruses. Some bacteria such as Wolbachia are currently being considered for the control of Dengue, Chikungunya and Zika.Yet little is known about the dynamics and diversity of Aedes-associated bacteria, including larval habitat features that shape their tempo-spatial distribution. We applied large-scale 16S rRNA amplicon sequencing to 960 adults and larvae of both Ae. aegypti and Ae. albopictus mosquitoes from 59 sampling sites widely distributed across nine provinces of Panama. We find both species share a limited, yet highly variable core microbiota, reflecting high stochasticity within their oviposition habitats. Despite sharing a large proportion of microbiota, Ae. aegypti harbours higher bacterial diversity than Ae. albopictus, primarily due to rarer bacterial groups at the larval stage. We find significant differences between the bacterial communities of larvae and adult mosquitoes, and among samples from metal and ceramic containers. However, we find little support for geography, water temperature and pH as predictors of bacterial associates. We report a low incidence of natural Wolbachia infection for both Aedes and its geographical distribution. This baseline information provides a foundation for studies on the functions and interactions of Aedes-associated bacteria with consequences for bio-control within Panama.