CdSe Quantum Dots for Two-Photon Fluorescence Thermal Imaging
CdSe Quantum Dots for Two-Photon Fluorescence Thermal Imaging
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DOI：
10.1021/nl1036098
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发表时间：
2010-12-01
期刊：
影响因子：
10.8
通讯作者：
Garcia Sole, Jose
中科院分区：
文献类型：
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作者：
Martinez Maestro, Laura;Martin Rodriguez, Emma;Garcia Sole, Jose
量子点的技术发展开创了荧光生物成像的新时代，这是随着新型多光子荧光显微镜的出现而推动的。在这里，潜在的使用CdSe量子点已被评估为双光子荧光显微镜的荧光纳米温度计。除了任何多光子激发过程所固有的空间分辨率的增强之外，双光子（近红外）激发导致发射强度的温度灵敏度比单光子（可见光）激发下实现的温度灵敏度高得多。峰值发射波长也是温度敏感的，为热成像提供了另一种方法，这对于纳米颗粒不均匀分散的系统特别有趣。基于双光子激发荧光的这些上级热敏特性，我们已经证明了CdSe量子点成像在生物相容性流体（磷酸盐缓冲盐水）中人工产生的温度梯度的能力，以及它们测量在单个活细胞中外部诱导的细胞内温度升高的能力。
The technological development of quantum dots has ushered in a new era in fluorescence bioimaging, which was propelled with the advent of novel multiphoton fluorescence microscopes. Here, the potential use of CdSe quantum dots has been evaluated as fluorescent nanothermometers for two-photon fluorescence microscopy. In addition to the enhancement in spatial resolution inherent to any multiphoton excitation processes, two-photon (near-infrared) excitation leads to a temperature sensitivity of the emission intensity much higher than that achieved under one-photon (visible) excitation. The peak emission wavelength is also temperature sensitive, providing an additional approach for thermal imaging, which is particularly interesting for systems where nanoparticles are not homogeneously dispersed. On the basis of these superior thermal sensitivity properties of the two-photon excited fluorescence, we have demonstrated the ability of CdSe quantum dots to image a temperature gradient artificially created in a biocompatible fluid (phosphate-buffered saline) and also their ability to measure an intracellular temperature increase externally induced in a single living cell.