Effects of exogenous putrescine on glycolysis and Krebs cycle metabolism in cucumber leaves subjected to salt stress
Effects of exogenous putrescine on glycolysis and Krebs cycle metabolism in cucumber leaves subjected to salt stress
复制标题
外源腐胺对盐胁迫下黄瓜叶片糖酵解和克雷布斯循环代谢的影响
DOI：
10.1007/s10725-015-0136-9
复制
发表时间：
2016-07-01
影响因子：
4.2
通讯作者：
Tang, Yuanyuan
中科院分区：
文献类型：
--
作者：
Zhong, Min;Yuan, Yinghui;Tang, Yuanyuan
研究了外源腐胺（8 mmol L-1）对黄瓜叶片碳水化合物含量、糖酵解代谢、柠檬酸循环中间产物、关键酶活性及其基因表达的影响。CV.以京优4号为材料，研究了盐胁迫（75 mmol L-1 NaCl）对水稻幼苗生长的影响。盐胁迫下叶片磷酸果糖激酶（PFK）、丙酮酸激酶（PK）和磷酸烯醇式丙酮酸激酶（PEPC）活性显著降低。NaCl胁迫导致PFK和PK基因表达量和丙酮酸含量显著下降，而PEPC基因表达量增加。盐胁迫还显著降低了异柠檬酸脱氢酶、苹果酸脱氢酶和琥珀酸脱氢酶的表达水平，导致叶片中柠檬酸、琥珀酸和苹果酸含量下降。外源腐胺逆转了盐胁迫，提高了糖酵解途径和Krebs循环中关键酶的基因表达，从而显著增加了有机酸和丙酮酸的产生量，促进了更多能量货币（ATP和ADP）的释放。说明Put有效参与糖酵解途径和Krebs循环，减少叶片中碳水化合物的过量积累，为防御盐胁迫提供更多的物质和能量，从而增强黄瓜幼苗对盐胁迫的耐受性。
The effects of exogenous putrescine (8 mmol L-1) on the carbohydrate content, glycolytic metabolism, citric acid cycle intermediates, key enzyme activities and their corresponding gene expression in cucumber (cucumis sativus L. cv. Jingyou NO.4) subjected to salt stress (75 mmol L-1 NaCl) in solution culture was studied. Phosphofructokinase (PFK), pyruvate kinase (PK) and phosphoenolpyruvate pyruvate kinase (PEPC) activities were significantly decreased in leaves when exposed to salt stress. NaCl stress caused the PFK and PK gene expression and the content of pyruvate to significantly decrease, while PEPC gene expression increased. Salt stress also significantly reduced isocitrate dehydrogenase, malate dehydrogenase and succinate dehydrogenase and their levels of transcription, causing decreases in the citric acid, succinic acid and malic acid content in leaves. Exogenous putrescine reversed the salt stress and increased gene expression and, in turn, the key enzymes involved in the glycolysis pathway and Krebs cycle, which significantly increased the amounts of organic acids and pyruvate produced, promoting the release of more energy currency (ATP and ADP). These results suggest that Put effectively participate glycolysis pathway and Krebs cycle, reducing the excessive accumulation of carbohydrates in the leaves, and providing more material and energy for the defense salt-induced injury, thus enhances cucumber seedling to salt stress tolerance.