Lichen Depsides and Depsidones Reduce Symptoms of Diseases Caused by Tobacco Mosaic Virus (TMV) in Tobacco Leaves
Lichen Depsides and Depsidones Reduce Symptoms of Diseases Caused by Tobacco Mosaic Virus (TMV) in Tobacco Leaves
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DOI：
10.1177/1934578x1200700514
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发表时间：
2012-05
影响因子：
1.8
通讯作者：
Ingrid Ramírez;Soledad Araya;M. Piovano;M. Carvajal;Álvaro Cuadros-Inostroza;Luis Espinoza;J. Garbarino
中科院分区：
文献类型：
--
作者：
Ingrid Ramírez;Soledad Araya;M. Piovano;M. Carvajal;Álvaro Cuadros-Inostroza;Luis Espinoza;J. Garbarino
对从地衣中分离出的两种缩酚醛和五种缩酚醛酮进行了测试，以确定它们对受到烟草花叶病毒 (TMV) 攻击的烟叶的体内保护作用。结果表明，大多数这些化合物能够减少病毒感染后坏死病变的数量和/或大小。 Pannarin、1′-氯-pannarin 和粘胶酸提供了更有效的保护效果，减少了至少 45% 的病变数量和大小。实时 PCR 检测通过检查参与植物防御机制的基因的反应行为来探索针对 TMV 的作用靶点。地衣物质的应用不会导致病原体相关基因 (PR1a)、丙二烯氧化物合酶 2 (AOS2) 或氧化植物二烯酸还原酶 (OPR3) 基因的转录水平发生变化。因此，在用地衣化合物处理的烟叶中观察到的保护可能是由不涉及SA或JA介导的防御性植物反应的机制介导的。提出了一种可能的结构-活性关系。
Two depsides and five depsidones, isolated from lichens, were tested to determine their in vivo protective effects on tobacco leaves challenged with Tobacco Mosaic Virus (TMV). The results indicate that most of these compounds are able to reduce either the number and/or the size of necrotic lesions following virus infection. Pannarin, 1′-chloro-pannarin and stictic acid provided the more effective protective results, reducing by at least 45% the number and size of lesions. Real Time PCR assays were used to explore the target of action against TMV by examining the response behavior of genes involved in the plant defense mechanism. The application of the lichen substances did not lead to changes in the transcriptional levels of pathogen-related (PR1a), allene oxide synthase 2 (AOS2) or oxophytodienoate reductase (OPR3) genes. Thus, the protection observed in the tobacco leaves treated with the lichen compounds may be mediated by a mechanism which does not involved the SA- or JA-mediated defensive plant response. A possible structure-activity relationship is presented.