Expression profiling of T-cell lymphomas differentiates peripheral and lymphoblastic lymphomas and defines survival related genes
Expression profiling of T-cell lymphomas differentiates peripheral and lymphoblastic lymphomas and defines survival related genes
复制标题
DOI：
10.1158/1078-0432.ccr-04-0269
复制
发表时间：
2004-08-01
影响因子：
11.5
通讯作者：
Benitez, J
中科院分区：
文献类型：
--
作者：
Martinez-Delgado, B;Meléndez, B;Benitez, J
目的：T细胞淋巴瘤是一种异质性和侵袭性的肿瘤，其致病性改变在很大程度上尚不清楚。表达谱分析已被证明是肿瘤分子分类的有用工具。实验设计：使用DNA微阵列（CNIO-OncoChip）包含6386个癌症相关基因，我们建立了T细胞淋巴瘤的表达谱，并将其与正常淋巴细胞和淋巴结进行比较。我们发现外周和淋巴母细胞T细胞淋巴瘤之间存在显著差异，其中包括核因子-κ B信号通路的失调。我们还确定了外周T细胞淋巴瘤肿瘤与正常T淋巴细胞或反应性淋巴结之间的差异表达基因，这些基因可能代表这些淋巴瘤的候选肿瘤标志物。此外，与生存相关的基因之间的密切关系和那些区分疾病的各个阶段和对therapeutic的反应被发现。结论：我们的研究结果反映了基因表达谱的价值，以深入了解T细胞淋巴瘤的发病机制中所涉及的分子改变。
Purpose: T-Cell lymphomas constitute heterogeneous and aggressive tumors in which pathogenic alterations remain largely unknown. Expression profiling has demonstrated to be a useful tool for molecular classification of tumors.Experimental Design: Using DNA microarrays (CNIO-OncoChip) containing 6386 cancer-related genes, we established the expression profiling of T-cell lymphomas and compared them to normal lymphocytes and lymph nodes.Results: We found significant differences between the peripheral and lymphoblastic T-cell lymphomas, which include a deregulation of nuclear factor-kappaB signaling pathway. We also identify differentially expressed genes between peripheral T-cell lymphoma tumors and normal T lymphocytes or reactive lymph nodes, which could represent candidate tumor markers of these lymphomas. Additionally, a close relationship between genes associated to survival and those that differentiate among the stages of disease and responses to therapy was found.Conclusions: Our results reflect the value of gene expression profiling to gain insight about the molecular alterations involved in the pathogenesis of T-cell lymphomas.