Intestinal polymeric immunoglobulin receptor is affected by type and route of nutrition.
Intestinal polymeric immunoglobulin receptor is affected by type and route of nutrition.
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DOI：
10.1177/0148607107031005351
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发表时间：
2007-09
期刊：
影响因子：
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通讯作者：
Y. Sano;F. E. Gomez;W. Kang;J. Lan;Y. Maeshima;J. Hermsen;C. Ueno;K. Kudsk;K. Kudsk
中科院分区：
文献类型：
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作者：
Y. Sano;F. E. Gomez;W. Kang;J. Lan;Y. Maeshima;J. Hermsen;C. Ueno;K. Kudsk;K. Kudsk
背景分泌型免疫球蛋白A(SIgA)可防止病原体黏附于粘膜表面，防止侵袭性感染。聚合免疫球蛋白受体(PIgR)位于上皮细胞的基底外侧表面，与固有层浆细胞产生的二聚体免疫球蛋白A(IgA)结合。这种IgA-pIgR复合体在顶端运输，在那里IgA以SIgA的形式排出到粘膜表面。我们先前的工作表明，与喂食食物或复合肠道饮食(CED)的小鼠相比，灌胃(IG，一种基本饮食模型)和静脉注射肠外营养(PN)溶液的小鼠肠道T和B细胞、SIgA和白介素4(IL-4)的水平都有所降低。先前的工作还表明，静脉注射PN喂养的小鼠，IgA向粘膜表面的转运减少。由于IL-4上调pIgR的产生，因此本工作研究了这些饮食对肠道pIgR的影响。方法将雄性肿瘤研究所(ICR)小鼠随机分为3组，每组11只，分别采用静脉留置导尿管喂养、胃造口行CED或IG PN治疗、静脉注射PN治疗5天。CED和PN是等热量、等氮的。黏膜冲洗后取小鼠小肠作pIgR和IL-4检测。用双抗体夹心法检测IgA和IL-4水平，用Western印迹法检测pIgR水平。结果静脉PN组和IG PN组小鼠小肠pIgR表达、IgA水平和IL-4水平均显著降低。结论肠道刺激不足影响肠道SIgA水平降低的多种机制，包括固有层T、B细胞减少，Th-2 IgA刺激细胞因子减少，IgA转运蛋白pIgR表达降低。
BACKGROUND Secretory immunoglobulin A (SIgA) prevents adherence of pathogens at mucosal surfaces to prevent invasive infection. Polymeric immunoglobulin receptor (pIgR) is located on the basolateral surface of epithelial cells and binds dimeric immunoglobulin A (IgA) produced by plasma cells in the lamina propria. This IgA-pIgR complex is transported apically, where IgA is exocytosed as SIgA to the mucosal surface. Our prior work shows that mice fed intragastric (IG, an elemental diet model) and IV parenteral nutrition (PN) solution have reduced intestinal T and B cells, SIgA, and interleukin-4 (IL-4) compared with mice fed chow or a complex enteral diet (CED). Prior work also demonstrates a reduction in IgA transport to mucosal surfaces in IV PN-fed mice. Because IL-4 up-regulates pIgR production, this work studies the effects of these diets on intestinal pIgR. METHODS Male Institute of Cancer Research (ICR) mice were randomized to chow (n = 11) with IV catheter, CED (n = 10) or IG PN (n = 11) via gastrostomy and IV PN (n = 12) for 5 days. CED and PN were isocaloric and isonitrogenous. Small intestine was harvested for pIgR and IL-4 assays after mucosal washing for IgA. IgA and IL-4 levels were analyzed by enzyme-linked immunosorbent assay and pIgR by Western blot. RESULTS Small intestinal pIgR expression, IgA levels, and IL-4 levels decreased significantly in IV PN and IG PN groups. CONCLUSIONS Lack of enteral stimulation affects multiple mechanisms responsible for decreased intestinal SIgA levels, including reduced T and B cells in the lamina propria, reduced Th-2 IgA-stimulating cytokines, and impaired expression of the IgA transport protein, pIgR.