Useful and Fast Method for Blood Lead and Cadmium Determination Using ICP-MS and GF-AAS; Validation Parameters
Useful and Fast Method for Blood Lead and Cadmium Determination Using ICP-MS and GF-AAS; Validation Parameters
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DOI：
10.1002/jcla.21826
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发表时间：
2016-03-01
影响因子：
2.7
通讯作者：
Janasik, Beata
中科院分区：
文献类型：
--
作者：
Trzcinka-Ochocka, Malgorzata;Brodzka, Renata;Janasik, Beata
背景在临床分析中，特别是血铅（Pb-B）和镉（Cd-B）的测定，方法的准确度和精密度至关重要。本文研究了电感耦合等离子体质谱（ICP-MS）测定铅硼和镉硼的方法（电感耦合等离子体质谱法）以及GF-AAS方法该方法的原理是基于通过加入5%的硝酸使血液样品脱蛋白，从而消除样品中蛋白质的存在，从而排除有机基质对结果测定的影响。比较了ICP-MS和GF-AAS测定铅作业工人血铅和镉的方法，结果表明ICP-MS和GF-AAS的验证参数基本一致，但ICP-MS测定铅的方法优于GF-AAS。ICP-MS和GF-AAS测定Pb-B的检出限（3xSD）分别为0.16和1.0g/l，Cd-B的检出限分别为0.08和0.02g/l。使用这两种技术，可比较的Pb-B和Cd-B测定的相关系数（rs）显示出非常好的统计学显著相关性，对于Pb-B为r = 0.9988，P < 0.0001，对于Cd-B为r = 0.9949，P < 0.0001，Cd-B.结论血液样品去蛋白法仍是消除光谱干扰和干扰的最佳方法。有机物详细的方法是特别致力于临床实验室和测定低浓度的铅和镉的生物样品。(C)2014 Wiley Periodicals，Inc.
BackgroundIn case of clinical analysis, especially in blood lead (Pb-B) and cadmium (Cd-B) determination, the accuracy and precision of the method are crucial. The objective of this article is to present a simple and useful method for Pb-B and Cd-B determination using ICP-MS (inductively coupled plasma-mass spectrometry) as well as GF-AAS (graphite furnace-atomic absorption spectrometry).MethodsThe principle of the method is based on the deproteinization of blood samples by addition of 5% nitric acid that eliminates the presence of the protein in the samples, thereby excluding the influence of the organic matrix on the result determinations. A comparison of the two techniques ICP-MS and GF-AAS was established for Pb and Cd determinations in the same 40 blood samples collected from lead workers.ResultsThe results showed that validation parameters for ICP-MS and GF-AAS were similar, however better for ICP-MS for Pb-B determinations. The detection limit (3xSD) for Pb-B determinations for ICP-MS and GF-AAS was, respectively, 0.16 and 1.0g/l, and for Cd-B it was, respectively, 0.08 and 0.02g/l. Correlation coefficients (rs) for comparable Pb-B and Cd-B determinations, using these two techniques, showed very good statistically significant correlations and were r = 0.9988, P < 0.0001 for Pb-B and r = 0.9949, P < 0.0001 for Cd-B.ConclusionsThe obtained results indicate that the method of deproteinization of blood samples is still the best way to eliminate spectral interferences and influence of the organic matter. The elaborated method is especially dedicated to clinical laboratories and determined low concentrations of lead and cadmium in biological samples. (C) 2014 Wiley Periodicals, Inc.