Adsorbed and free lipid bilayers at the solid-liquid interface
Adsorbed and free lipid bilayers at the solid-liquid interface
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DOI：
10.1007/s100510050725
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发表时间：
1999-04-01
影响因子：
1.6
通讯作者：
Graner, F
中科院分区：
文献类型：
--
作者：
Charitat, T;Bellet-Amalric, E;Graner, F
制备了吸附在硅-水界面上的单、双磷胆碱(DPPC和DSPC)双层膜，并对其进行了表征。第二个双层被称为“自由双层”，是一种新型的高水合体系，漂浮在第一个双层之上20~30埃。由于Langmuir-Blodgett和Langmuir-Schaeffer技术的结合，稳健和可重复的制备成为可能。经过精心优化的中子反射率测量使人们能够准确、无损地表征涂层的结构、水合作用和粗糙度。这项工作为研究膜脂和可溶性蛋白质之间的相互作用开辟了新的可能性，特别是太小而用其他技术看不见的多肽。
Single and double phosphocholine (DPPC and DSPC) bilayers adsorbed at the silicon-water interface have been prepared and characterised. The second bilayer, called "free bilayer", is a novel highly hydrated system floating at 20 to 30 Angstrom above the first one. Robust and reproducible preparation has been possible thanks to a combination of Langmuir-Blodgett and Langmuir-Schaeffer techniques. Carefully optimised neutron reflectivity measurements have allowed a precise non-destructive characterisation of the structure, hydration and roughness of the layers. This work opens new possibilities for the investigation of the interaction between membrane lipids and soluble proteins, in particular peptides too small to be Visible with other techniques.