Transcription analyses of GmICHG, a gene coding for a b-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr
Transcription analyses of GmICHG, a gene coding for a b-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr
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GmICHG 的转录分析，GmICHG 是一种编码 b-葡萄糖苷酶的基因，可催化大豆中异黄酮缀合物的特异性水解
DOI：
10.1016/j.plantsci.2013.03.006
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发表时间：
2013
期刊：
影响因子：
5.2
通讯作者：
DongChan Yoo
中科院分区：
文献类型：
--
作者：
S.Ahmad;O.Keskin;K.Mizuguchi;A.Sarai;R.Nussinov;DongChan Yoo
大豆异黄酮偶联物水解型β-葡萄糖苷酶[MERR.]催化异黄酮偶联物(异黄酮类的β-7-O-(丙二酰基)葡萄糖苷)的特定水解，以产生游离异黄酮类。本研究对大豆幼苗各器官GmICHG转录水平的变化进行了研究。Enrei)对微生物侵染和非生物胁迫的响应进行了分析，并与编码2-羟基异黄酮合酶(GmIFS)和异黄酮7-O-葡萄糖基转移酶(GmIF7GT)的基因进行了比较。GmICHG最初只在根中大量表达，在其他器官中只有少量表达。大豆疫霉侵染根部后，GmICHG在根部和其他器官中的转录受到抑制。在子叶受伤时，根中GmICHG转录出现短暂的长距离上调，而在子叶中受到真菌感染时，根中GmICHG转录的上调出现延迟，在感染后10h达到最大值。GmIFs和GmIF7GT均未观察到这种远距离上调模式。慢生根瘤菌处理后，GmICHG的转录水平基本保持不变。GmICHG在根中的转录对根的各种胁迫也很敏感，如淹水、酵母膏诱导、干旱以及植物激素如脱落酸、水杨酸、茉莉酸和乙烯的处理。
Isoflavone conjugate-hydrolyzing β-glucosidase (GmICHG) of soybeans [Glycine max (L.) Merr.] catalyzes the specific hydrolysis of isoflavone conjugates (β-7-O-(malonyl)glucosides of isoflavones) to produce free isoflavones. In this study, changes in the transcription levels of GmICHG in the individual organs of soybean seedlings (cv. Enrei) in response to microbial infection and abiotic stresses were analyzed and compared with those of genes coding for 2-hydroxyisoflavanone synthase (GmIFS) and isoflavone 7-O-glucosyltransferase (GmIF7GT). GmICHG was originally expressed in abundance only in the roots and at low levels only in the other organs. The transcription of GmICHG in the roots and other organs was suppressed upon infection of the roots by Phytophthora sojae. Upon wounding of the cotyledon, a transient long-distance up-regulation of GmICHG transcription in the roots was observed; upon fungal infection in the cotyledon, however, a delayed elevation of GmICHG transcription took place in the roots with the maximum at 10h after the infection. Such long-distance up-regulation patterns were not observed with either GmIFS or GmIF7GT. The transcription levels of GmICHG remained essentially unchanged upon treatment of the roots with Bradyrhizobium japonicum. The transcription of GmICHG in the roots was also sensitive to a variety of stresses on the roots, such as flooding, elicitation with yeast extract, drought, and treatment with plant hormones such as abscisic, salicylic, and jasmonic acids and ethylene.