Measurement of Antibody-Mediated Reduction of Plasmodium yoelii Liver Burden by Bioluminescent Imaging.
Measurement of Antibody-Mediated Reduction of Plasmodium yoelii Liver Burden by Bioluminescent Imaging.
复制标题
通过生物发光成像测量抗体介导的约氏疟原虫肝脏负担的减少。
DOI：
10.1007/978-1-4939-2815-6_6
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Kappe,StefanHI
中科院分区：
文献类型：
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作者：
Sack,BrandonK;Miller,JessicaL;Vaughan,AshleyM;Kappe,StefanHI
针对疟疾的感染性子孢子阶段的抗体已被证明在预防肝脏感染和减轻随后的血液阶段方面是有效的。然而，只有少数抗体靶点已被审查并显示出成功介导体内保护。更有限的是测量抗体如何有效地减少在肝脏中建立感染的寄生虫数量的方法。传统上，只有感染小鼠肝脏的qPCR才能准确测量肝脏寄生虫负荷。然而，该程序需要处死动物，并且排除了对随后的血液阶段感染的监测。在这里，我们描述了一种方法，准确地评估抗体介导的减少寄生虫肝负荷相结合的被动或主动免疫的小鼠和蚊子叮咬的挑战与表达转基因P。约氏寄生虫。该方法快速、可靠，可在同一动物中观察血液期疾病。随着对更有效的疟疾疫苗的研究继续进行，该模型将被证明是筛选新型抗体靶点功效的组成部分。
Antibodies against the infectious sporozoite stage of malaria have been shown to be effective in preventing infection of the liver and in mitigating the ensuing blood stage. However, only a handful of antibody targets have been vetted and shown to be successful in mediating in vivo protection. Even more limited are the means with which to measure how effectively antibodies can reduce the number of parasites establishing infection in the liver. Traditionally, only qPCR of infected mouse livers could accurately measure liver parasite burden. However, this procedure requires sacrifice of the animal and precludes monitoring of the ensuing blood stage infection. Herein we describe a method of accurately assessing antibody-mediated reduction of parasite liver burden by combining passive or active immunization of mice and mosquito bite challenge with luciferase-expressing transgenicP. yoeliiparasites. This method is rapid, reliable and allows for observation of blood stage disease in the same animal. This model will prove integral in screening the efficacy of novel antibody targets as the search for a more effective malaria vaccine continues.