Preparation of chromatin assembly extracts from preblastoderm Drosophila embryos.
Preparation of chromatin assembly extracts from preblastoderm Drosophila embryos.
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从前胚层果蝇胚胎中制备染色质组装提取物。
DOI：
10.1007/978-1-59745-190-1_1
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发表时间：
2009
影响因子：
--
通讯作者：
P. Becker
中科院分区：
文献类型：
--
作者：
E. Bonte;P. Becker
对染色质结构和功能进行严格的生化分析需要在体外组装染色质。通过盐梯度透析从纯组蛋白或重组组蛋白中重组核小体阵列的一个有用的替代方法是在生理条件下从组装提取物中组装更复杂的染色质。由于存在大量的组蛋白、组蛋白伴侣和atp依赖的核小体间隔因子的天然复合物，前胚层胚胎的提取物已被证明是特别有效的。所得的染色质是体内生理染色质的极好近似。本章描述了染色质组装提取物的制备和染色质组装反应。
A rigorous biochemical analysis of chromatin structure and function requires the assembly of chromatin in vitro. A useful alternative to reconstituting nucleosomal arrays from pure or recombinant histones by salt gradient dialysis is the assembly of more complex chromatin from assembly extracts under physiological conditions. Extracts from preblastoderm embryos have proven to be particularly efficient, due to the presence of large stores of native complexes of histones, histone chaperones and ATP-dependent nucleosome spacing factors. The resulting chromatin is an excellent approximation of physiological chromatin in vivo. This chapter describes the preparation of chromatin assembly extracts and the chromatin assembly reaction.