Activin and inhibin in the human adrenal gland. Regulation and differential effects in fetal and adult cells.
Activin and inhibin in the human adrenal gland. Regulation and differential effects in fetal and adult cells.
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人类肾上腺中的激活素和抑制素。
DOI：
10.1172/jci115827
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Jaffe,RB
中科院分区：
文献类型：
--
作者：
Spencer,SJ;Rabinovici,J;Mesiano,S;Goldsmith,PC;Jaffe,RB
最近的实验数据表明，激活素和激活素在发育中发挥关键作用。作为我们对人胎儿肾上腺生长和分化的研究的一部分，我们研究了亚基定位，以及人胎儿和成人肾上腺中激活素和β-肾上腺素的促有丝分裂和类固醇生成作用。在胎儿和成人肾上腺皮质中检测到所有三种激活素和β-腺苷酸亚基蛋白（α、β A和β B）。免疫反应性激活素A二聚体在妊娠中期胎儿和新生儿肾上腺中被证明。ACTH 1 -24刺激胎儿肾上腺细胞α和β A亚单位信使RNA的表达。此外，ACTH引起胎儿和成人肾上腺细胞分泌的免疫反应性α亚单位水平上升。人重组激活素A抑制有丝分裂和增强ACTH刺激的皮质醇分泌培养的胎儿区细胞，但不确定区或成人肾上腺细胞。重组拟南芥素-A没有明显的促有丝分裂或类固醇生成作用。因此，激活素选择性地抑制胎区增殖，并增强ACTH诱导的胎区类固醇产生的皮质醇/硫酸脱氢表雄酮比率的变化。这些数据表明激活素A可能是一种受ACTH调节的自分泌或旁分泌因子，参与调节人胎儿肾上腺的生长和分化功能。图片
Recent experimental data have revealed that activins and inhibins exert pivotal effects on development. As part of our studies on growth and differentiation of the human fetal adrenal gland, we examined the subunit localization, as well as the mitogenic and steroidogenic actions of activin and inhibin in human fetal and adult adrenals. All three activin and inhibin subunit proteins (alpha, beta A, and beta B) were detected in the fetal and adult adrenal cortex. Immunoreactive activin-A dimer was demonstrated in midgestation fetal and neonatal adrenals. ACTH1-24-stimulated fetal adrenal cell expression of alpha and beta A subunit messenger RNA. In addition, ACTH elicited a rise in levels of immunoreactive alpha subunit secreted by fetal and adult adrenal cells. Human recombinant activin-A inhibited mitogenesis and enhanced ACTH-stimulated cortisol secretion by cultured fetal zone cells, but not definitive zone or adult adrenal cells. Recombinant inhibin-A had no apparent mitogenic or steroidogenic effects. Thus, activin selectively suppressed fetal zone proliferation and enhanced the ACTH-induced shift in the cortisol/dehydroepiandrosterone sulfate ratio of fetal zone steroid production. These data indicate that activin-A may be an autocrine or paracrine factor regulated by ACTH, involved in modulating growth and differentiated function of the human fetal adrenal gland.Images