MARKER PROFILES OF HUMAN-LEUKEMIA AND LYMPHOMA CELL-LINES
MARKER PROFILES OF HUMAN-LEUKEMIA AND LYMPHOMA CELL-LINES
复制标题
DOI：
10.1007/bf00405069
复制
发表时间：
1981-01-01
影响因子：
3.6
通讯作者：
OHNUMA, T
中科院分区：
文献类型：
--
作者：
MINOWADA, J;KOSHIBA, H;OHNUMA, T
应用多标记分析法对55株人白血病淋巴瘤细胞系（包括15株T细胞、30株B细胞、4株骨髓单核细胞和6株非T、非B细胞）进行了标记分析。使用的多种标记物包括通过玫瑰花结或免疫荧光试验、酶测定、细胞遗传学分析和功能测定检测的许多细胞表面标记物。所有细胞系均为ALL（急性淋巴细胞性白血病）、AM（粒单核细胞性白血病）、C（慢性）LL、CML（急变期）等原发性白血病-淋巴瘤或各种淋巴瘤。单克隆性，在分化成熟的特定阶段的冷冻状态，和细胞遗传学标记在每个白血病淋巴瘤细胞系显着的共同特性，多年的培养稳定。对344例未经培养的新鲜白血病-淋巴瘤进行多标记分析，发现其一般特征相似，甚至不完全相同。虽然没有发现任何类型肿瘤的特异性标志物，但该研究为更好地理解白血病-淋巴瘤的生物学和深入了解人类正常造血细胞分化提供了基础。
By means of multiple marker analysis, 55 human leukemia-lymphoma cell lines including 15 T-cell, 30 B-cell, 4 myelomonocytic-cell and 6 non-T, non-B cell lines were characterized for their marker profiles. The multiple markers used included a number of cell surface markers as detected by rosette or immunofluorescence tests, enzyme assays, cytogenetic analysis and functional assays. All the cell lines represented original leukemia-lymphoma of ALL [acute lymphocytic leukemia], AM[myelomonocytic]L, C[chronic]LL, CML in blastic phase or a variety of lymphomas. The monoclonality, a frozen state at a specific stage of differentiation-maturation, and cytogenetic marker in each leukemia-lymphoma cell line were remarkable common properties, stable for years of cultivation. Similar, if not identical, general characteristics were observed in the study on 344 cases of uncultured fresh leukemia-lymphomas by the multiple marker analysis. While no single marker specific to any type of tumor was found, the study offers a basis for better understanding of the biology of leukemia-lymphoma and an insight into normal hematopoietic cell differentiation in man.