Epidemiology of toxoplasmosis in white tailed deer (Odocoileus virginianus): Occurrence, congenital transmission, correlates of infection, isolation, and genetic characterization of Toxoplasma gondii
Epidemiology of toxoplasmosis in white tailed deer (Odocoileus virginianus): Occurrence, congenital transmission, correlates of infection, isolation, and genetic characterization of Toxoplasma gondii
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DOI：
10.1016/j.vetpar.2014.01.006
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发表时间：
2014-05-28
影响因子：
2.6
通讯作者：
Su, C.
中科院分区：
文献类型：
--
作者：
Dubey, J. P.;Dennis, P. M.;Su, C.
弓形虫病在白色尾鹿（WTD）中的流行率很高，但对弓形虫病在这种宿主中的流行病学知之甚少。在本研究中，我们比较了T. 2012年和2013年从俄亥俄州的大都会公园收集的749只WTD和2008年、2009年和2010年在明尼苏达州射杀的487只WTD鹿的弓形虫血清阳性率。检测血清样本中的T抗体。用改良凝集试验（临界滴度，25）检测弓形虫。此外，将来自俄亥俄州的123份血清阳性WTD的心肌样品在胃蛋白酶中消化，并对这些样品进行生物测定以分离T。刚地。此外，为了估计经胎盘传播率，在小鼠中对来自明尼苏达州的148只鹿的155只胎儿（包括双胞胎）的脑进行生物测定，以分离活的T。刚地。T.不同年份、不同地域、不同年龄鹿的弓形虫感染率不同。在2012年和2013年采样的俄亥俄州鹿中，两年的血清阳性率相似（分别为73.4%和75.7%）;值得注意的是，227只鹿中有150只（66.1%）
The prevalence of Toxoplasma gondii in white tailed deer (WTD) in the USA is high but little is known of the epidemiology of toxoplasmosis in this host. In the present study, we compared T. gondii seroprevalence from 749 WTD collected in 2012 and 2013 from a Metropolitan Park in Ohio and 487 WTD deer shot in Minnesota during 2008, 2009, and 2010. Serum samples were tested for antibodies to T. gondii by the modified agglutination test (cut-off titer, 25). Additionally myocardial samples from 123 seropositive WTD from Ohio were digested in pepsin and the digests were bioassayed for the isolation of T. gondii. Furthermore, to estimate transplacental rate of transmission, brains from 155 fetuses (included twins) from 148 deer from Minnesota were bioassayed in mice for the isolation of viable T. gondii. Seroprevalence of T. gondii varied with the year of collection, geography, and the age of deer. Of the Ohio deer sampled in 2012 and 2013 seroprevalences for the two years were similar (73.4% and 75.7%, respectively); remarkably 150 (66.1%) of 227 deer of