GROUP-SPECIFIC SERUM ANTIBODY-RESPONSES IN CHILDREN WITH PRIMARY AND RECURRENT RESPIRATORY SYNCYTIAL VIRUS-INFECTIONS
GROUP-SPECIFIC SERUM ANTIBODY-RESPONSES IN CHILDREN WITH PRIMARY AND RECURRENT RESPIRATORY SYNCYTIAL VIRUS-INFECTIONS
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DOI：
10.1093/infdis/164.1.15
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发表时间：
1991-07-01
影响因子：
6.4
通讯作者：
MURPHY, BR
中科院分区：
文献类型：
--
作者：
MUELENAER, PM;HENDERSON, FW;MURPHY, BR
研究了在研究日托中心从婴儿早期开始纵向随访的儿童对第一次和第二次呼吸道合胞病毒（RSV）感染的抗原组特异性血清抗体应答。 使用A组和B组原型RSV毒株的抗原进行了噬斑减少中和（PRN）抗体测定以及融合（F）和附着（G）糖蛋白的ELISA。 比较了对与感染病毒株的抗原组同源和异源的病毒的抗原的应答。 原发性A组感染在PRN（B）和G（B）和F（B）的ELISA中引发与B组病毒交叉反应的抗体。 相反，仅在F（A）ELISA中，初次B组感染诱导与A组病毒交叉反应的抗体平均浓度显著增加。 在有原发性A组病毒感染史的儿童中，由B组病毒引起的第二次RSV感染诱导PRN（A）和G（A）测定的异源性升高，表明既往A组病毒感染在第二次RSV感染B组病毒时已引发更广泛的交叉反应性抗体应答。
Antigenic group-specific serum antibody responses to first and second respiratory syncytial virus (RSV) infections were studied in children who had been followed longitudinally from early infancy in a research day-care center. Plaque-reduction neutralizing (PRN) antibody assays and ELISAs for the fusion (F) and attachment (G) glycoproteins were done using antigens of prototype RSV strains from groups A and B. Responses to antigens of viruses homologous and heterologous to the antigenic group of the infecting viral strain were compared. Primary group A infection elicited antibodies cross-reactive with group B virus in the PRN(B) and the ELISAs for G(B) and F(B). In contrast, primary group B infection induced significant increases in mean concentrations of antibody cross-reactive with group A virus only in the F(A) ELISA. Second RSV infections caused by group B viruses in children with histories of primary group A infection induced heterologous rises in the PRN(A) and G(A) assays, suggesting that prior group A infection had primed for a more extensive cross-reacting antibody response at the time of second RSV infections with group B viruses.