Quantifying the Traction Force of a Single Cell by Aligned Silicon Nanowire Array
Quantifying the Traction Force of a Single Cell by Aligned Silicon Nanowire Array
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DOI：
10.1021/nl901774m
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发表时间：
2009-10-01
期刊：
影响因子：
10.8
通讯作者：
Wang, Zhong Lin
中科院分区：
文献类型：
--
作者：
Li, Zhou;Song, Jinhui;Wang, Zhong Lin
静止细胞的形态、运动、粘附、贴壁、侵袭和转移等物理行为可能是决定其生物学特性的重要因素。哺乳动物细胞物理特性的变化可能是疾病的征兆。在本文中，我们提出了一种基于硅纳米线阵列的技术，用于量化代表三个不同群体的单细胞的机械行为：正常哺乳动物细胞、良性细胞（L929）和恶性细胞（HeLa）。通过在NW阵列顶部培养细胞，通过定量分析纳米线的弯曲，测量了两种不同肿瘤细胞（HeLa，L929）的最大牵引力。癌细胞表现出比正常细胞大的牵引力，对于HeLa细胞为20%，对于L929细胞为50%。已经测量了作为培养时间的函数的L929细胞和机械细胞的牵引力。探讨了细胞伸展面积与细胞牵引力的关系。我们的研究可能是重要的研究单细胞的力学性能和迁移特性，可能提供一个新的细胞水平的诊断技术。
The physical behaviors of stationary cells, such as the morphology, motility, adhesion, anchorage, invasion and metastasis, are likely to be important for governing their biological characteristics. A change in the physical properties of mammalian cells could be an indication of disease. In this paper, we present a silicon-nanowire-array based technique for quantifying the mechanical behavior of single cells representing three distinct groups: normal mammalian cells, benign cells (L929), and malignant cells (HeLa). By culturing the cells on top of NW arrays, the maximum traction forces of two different tumor cells (HeLa, L929) have been measured by quantitatively analyzing the bending of the nanowires. The cancer cell exhibits a larger traction force than the normal cell by similar to 20% for a HeLa cell and similar to 50% for a L929 cell. The traction forces have been measured for the L929 cells and mechanocytes as a function of culture time. The relationship between cells extending area and their traction force has been Investigated. Our study is likely important for studying the mechanical properties of single cells and their migration characteristics, possibly providing a new cellular level diagnostic technique.