Modifications of serotonergic and adrenergic receptor concentrations in the brain of aggressive Canis familiaris
Modifications of serotonergic and adrenergic receptor concentrations in the brain of aggressive Canis familiaris
复制标题
DOI：
10.1016/j.cbpb.2004.09.019
复制
发表时间：
2004-11-01
影响因子：
2.3
通讯作者：
Re, G
中科院分区：
文献类型：
--
作者：
Badino, P;Odore, R;Re, G
本研究的目的是测量正常和攻击性犬不同脑区（额叶皮质、海马、下丘脑和丘脑）的β-肾上腺素能（β-AR）和β-肾上腺素能（5-HTR）受体浓度。本研究使用了八只成年雄性狗，年龄为4.2+/-0.6岁，除攻击性外没有表现出任何临床症状。将8只健康雄性犬（4.4+/-0.8岁）用作对照，这些犬无神经和/或行为障碍和意外死亡史。安乐死后收集整个额叶皮质、海马、丘脑和下丘脑，并通过超离心获得质膜组分。分别使用特异性放射性配体[（-）[H-3]CGP 12177和5-羟基[H-3]-色胺三氟乙酸盐]，通过结合试验测定β-AR和5-HTR。在额叶皮质（P=0.001）、海马（P
The aim of the study was to measure beta-adrenergic (beta-AR) and serotonergic (5-HTR) receptor concentrations in different brain areas (frontal cortex, hippocampus, hypothalamus and thalamus) of normal and aggressive dogs. Eight adult male dogs, 4.2+/-0.6 years old, showing no clinical signs but aggression, were used for the study. Eight healthy male dogs, 4.4+/-0.8 years old, with no history of neurological and/or behavioural disorders and accidental death, were used as controls. The whole frontal cortex, hippocampus, thalamus and hypothalamus were collected after euthanasia and plasma membrane fractions obtained by ultracentrifiagation. beta-AR and 5-HTR were measured by binding assays using specific radioligand [(-)[H-3]CGP 12177 and 5-hydroxy[H-3]-tryptamine trifluoroacetate, respectively]. A significant decrease in beta-AR levels was observed in the frontal cortex (P=0.001), hippocampus (P