ENUMERATION OF TUBERCLE-BACILLI IN SPUTUM OF PATIENTS WITH PULMONARY TUBERCULOSIS
ENUMERATION OF TUBERCLE-BACILLI IN SPUTUM OF PATIENTS WITH PULMONARY TUBERCULOSIS
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DOI：
10.1128/aac.4.2.94
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发表时间：
1973-01-01
影响因子：
4.9
通讯作者：
JONES, JM
中科院分区：
文献类型：
--
作者：
HOBBY, GL;HOLMAN, AP;JONES, JM
描述了一种精确显微镜计数肺结核患者痰中抗酸杆菌的方法。在化疗前和/或化疗期间收集的 28 名患者的 269 份标本中，测定了抗酸杆菌总数和每毫升痰中可培养的结核杆菌细胞单位数，以确定该程序用于评估化疗效果的有用性。在治疗之前或治疗过程的早期，28 名患者中有 27 名的显微镜和培养可测量的杆菌之间存在良好的一致性。此后，五名患者出现系统性差异，而其他大多数患者则偶尔出现差异。然而，总的来说，在 28 名患者中的 20 名患者中，显微镜确定的群体提供了对存在的可培养结核杆菌数量的有用测量。尽管显微镜程序不允许对分枝杆菌进行物种鉴定或区分活细胞和非活细胞，但它通常可以在数小时而不是数天或数周内提供有关化疗期间痰中抗酸杆菌残留量的有用信息。讨论了那些显微镜检查未能提供有用信息的患者的疾病性质。
A method for precise microscopic enumeration of acid-fast bacilli in the sputum of patients with pulmonary tuberculosis is described. The total number of acid-fast bacilli and the number of culturable cell units of tubercle bacilli per milliliter of sputum were determined in 269 specimens from 28 patients collected prior to and/or during chemotherapy, to establish the usefulness of the procedure for evaluation of the effects of chemotherapy. Prior to or very early in the course of therapy, there was good agreement between microscopically and culturally measurable bacilli in 27 of 28 patients. Thereafter, there were systematic divergences in five patients and occasional divergences in most others. Yet, in general, in 20 of the 28 patients, the microscopically determined population provided a useful measure of the number of culturable tubercle bacilli present. Although the microscopic procedure does not allow species identification of mycobacteria or differentiation between viable and nonviable cells, it often provided, within hours rather than days or weeks, useful information on the residual burden of acid-fast bacilli in sputum during chemotherapy. The nature of the disease in those patients in whom microscopy failed to provide useful information is discussed.